Anti-S. aureus Protein A Aptamer(Cat#: CTMM-0124-TT23)
This product is an aptamer which binds to the S. aureus Protein A with an affinity of 1-50 nM.
Specific Inquiry 
Specifications
| Target Category |
Protein |
| Target |
Protein A |
| Target Overview |
Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs. It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. |
| Type |
DNA |
| Species Reactivity |
Bacteria |
| Length |
50-100 nt |
| Affinity (Kd) |
1-50 nM |
| Binding Conditions/Buffer |
100 mM NaCl, 20 mM Tris-HCl, 10 mM MgCl₂, 5 mM KCl, 1 mM CaCl₂, 0.005% Tween 20, pH 7.6 |
| GC Content |
50.00% |
| Molecular Weight |
18032.77 g/mole |
| nmoles/OD260 |
1.76 |
For Research Use Only. Not for Diagnostic or Therapeutic Applications.
