Background Services Overview Case Study Related Products Q&A Resources
What is ELONA?
Enzyme-linked immunosorbent assay (ELISA) is a fast and easy-to-use method for target detection through immunologic
reactions and enzyme immunoassay, which has been commonly applied for preclinical and clinical quantification for
several decades. Recently, a novel and improved ELISA, named enzyme-linked oligonucleotide assay (ELONA), has drawn
our attention, showing high accuracy and low batch differences with relatively lower cost.
Similarly, ELONA relies on aptamers, a class of single-stranded oligonucleotides with certain spatial structures
that can recognize targets with high specificity and affinity, to detect the target based on the principle of ELISA.
ELONA generally comprises several variations according to the difference of aptamer or antibody/aptamer.
Fig.1 Direct ELONA.1
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Fig.2 Aptamer sandwich ELONA.1
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Fig.3 Antibody-aptamer sandwich ELONA. (powered by APIS)
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Fig.4 Sandwich ELONA in aptamer-target-aptamer.2
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Fig.5 Sandwich ELONA in aptamer-target-antibody.2
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Fig.6 Sandwich ELONA in antibody-target-aptamer.2
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What Can We Do About ELONA?
A number of aptamers have been developed through in vitro systematic evolution of ligands by exponential
enrichment (SELEX) owing to their excellent performances, such as stable structure, high affinity (pM-nM), high
specificity, and lower batch-to-batch variations. Creative Biolabs established a comprehensive SELEX platform
several years ago, which has successfully fulfilled many projects. Based on our off-the-shelf aptamers products and perfect SELEX platform, our professional team provides custom
development services for ELONA kits and ELONA-based detect assays. Our tailored development services can be
delivered in a one-stop package, and also be a part of assay according to the customer's needs.
Our ELONA Development Process
Screening & selection
Screen and select the candidate aptamer with high specificity and proper modifications. The lead time will be
shorter if your desired aptamer is ready to use on our web. And, to be more convinced, a special antibody used
as a
control will be included. Also, the appropriate target concentration should be confirmed.
Matrix compatibility
When the aptamer is ready, sample preparation condition evaluation and exploration of linearity or matrix impact
on
blank measurement are needed to ensure the accuracy of detection and eliminate some unnecessary interference
factors.
Process optimization
An ELONA is beginning to take shape after a round of screening and exploration. What we need to do next is to
optimize the conditional and process, such as control settings, calibrator concentrations, incubation time,
incubation temperature, aptamer concentrations, and modifications.
Assay optimization and robustness
A seasoned ELONA will be generated after assessments on repeatability, multiplexing, cross-reactivity,
formulation,
design specification, SOP applicability, and some others.
Fig. 7 Major ELONA
development process. (Powered by APIS)
Why Choose Us for Your ENOAL Development?
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Custom SELEX development against a wide range of biomarkers or cells
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Different aptamer products are available based on your needs
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Extensive experience in ancillary techniques and services
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Fully customizable experimental design to meet customers' needs
Case Study
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A representative display of our optimized ELONA development process.
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Fig. 8 Screening & selection. (Powered by APIS)
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Step 1: Screening & selection.
Aptamer candidates were obtained by in vitro SELEX services. Immunoassays are performed to
compare the performance of different aptamer candidates (Clickmer 1, 2, 3 in Fig. 8) with three
concentrations of a target protein.
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Fig. 9 Matrix compatibility. (Powered by APIS)
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Step 2: Matrix compatibility tests.
Test the impact of different matrix concentrations on the assay signal by sandwich immunoassays.
Fig. 9 shows the detection effects of target protein in different matrices, including no serum,
12.5% serum, 25% serum, 50% serum, and 50% serum no target.
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Fig. 10 Process optimization. (Powered by APIS)
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Step 3: Process optimization.
Sandwich immunoassays were performed to find out the best conditions (incubation time and
temperature) for ELONA.
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Fig. 11 Assay optimization and robustness. (Powered by APIS)
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Step 4: Assay optimization and robustness.
A series of immunoassays were performed to characterize the analytical performance of the ELONA.
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Hot ELONA Kit Products
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Cat No
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Product Name
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Species Reactivity
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Assay Type
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CTMM1-TT-1
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SARS-CoV-2 IgG ELONA Kit
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Human
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Aptamer-based ELISA
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CTMM1-TT-2
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TBEV NS1 ELONA Kit
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Multiple species
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Aptamer-based ELISA
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Aptamer has been more and more often used in biological research and diagnostic assays. As a pioneer in drug
discovery, Creative Biolabs is proud to provide our clients with ELONA development services due to
our extensive experience and professional Research & Development team. To work side-by-side with you to develop
the kits or assays you need, please contact us for more information.
Resources
References
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Shola David, Michael, and Damira Kanayeva. "Enzyme linked oligonucleotide assay for the sensitive detection of
SARS-CoV-2 variants." Frontiers in Cellular and Infection Microbiology 12 (2022): 1017542.
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Dreymann, Nico, et al. "Aptamer-Based Sandwich Assay Formats for Detection and Discrimination of Human High-and
Low-Molecular-Weight uPA for Cancer Prognosis and Diagnosis." Cancers 14.21 (2022): 5222.
Questions & Answer
A: The turnaround time varies depending on specific requirements of the development process. However, we endeavor to provide a fast and accurate development schedule after detailed discussion with the customers.
A: We offer extensive after-service support including technical guidance for implementing the developed assay and troubleshooting.
A: Absolutely! We understand that different research requires different needs and hence offer customization options to cater to unique demands.
A: Yes, this service is highly generic. Aptamers can be developed against a broad range of targets, ranging from small molecules to complex proteins and even cells.
A: Standard storage conditions for ELONA assays apply. However, the specifics can vary depending on the nature of the aptamers and the target molecules. We'll provide you with detailed storage instructions upon completion of the service.
A: Yes, we can develop multiplex assays that can simultaneously detect and quantify multiple targets in a single run.
For Research Use Only.
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