Aptamers are oligonucleotide entities binding selectively to specific target molecules. With multiple advantages, they find diverse applications in analytics and medical domains. However, the intrinsic physicochemical properties of nucleic acid aptamers, encompassing vulnerability to nuclease degradation, swift renal clearance, and more, impose limitations on their use in disease diagnosis and treatment. Efforts to overcome these drawbacks necessitate aptamer modifications in their developmental trajectory.
Creative Biolabs stands at the forefront of aptamer discovery and modifications. Leveraging cutting-edge technologies and extensive proficiency in aptamer advancement, we proffer a comprehensive suite of aptamer modification services on a global scale. Our track record includes successful resolution of intricate aptamer modification endeavors, fostering substantial expertise. We tailor optimal modification strategies for enhancing aptamer performance, aligning with the distinct requisites of our clientele.
Fig. 1 Chemical modification strategies for nucleic acid aptamers.1
Aptamer Modifications Services in Creative Biolabs
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SERVICE ITEM
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DESCRIPTION
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2' Fluoro Modification Aptamer
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Modifying RNA or DNA aptamers with 2'-fluoro-substituted pyrimidines has demonstrated enhanced resistance against nucleases. Consequently, the chemical modification of aptamers using 2'-F-pyrimidines becomes imperative during aptamer development. Diverse strategies are offered by us to facilitate aptamer alteration utilizing 2'-amino pyrimidines, spanning library design, selection process amplification, and the chemical synthesis stages.
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2' NH2 Modification Aptamer
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Introducing 2'-NH2 modification stands as a prevalent alteration within the ribose moiety of the sugar-phosphate backbone. Aptamers incorporating this 2'-NH2 modification exhibit heightened nuclease stability relative to their unmodified counterparts. We provide extended 2'-NH2 modification services, elevating aptamer stability while preserving its intrinsic attributes.
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2' O-methyl (OCH3) Modification Aptamer
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The introduction of 2'-O-methyl modification entails substituting the 2'-hydroxyl group with 2'-O-methyl. Integration of 2'-O-methyl nucleotides into aptamers enhances their resistance to nucleases.
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3' Inverted Thymidine Modification Aptamer
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The integration of inverted thymidine at the 3'-terminus stands as a modification aimed at bolstering aptamer stability against nucleases. In comparison to unmodified and 3'-biotin-modified aptamers, the inclusion of 3'-inverted thymidine substantially augments aptamers' resilience to nuclease degradation. modifications.
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Fluorescence Modification Aptamer
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Fluorescent modification methodologies find extensive utility in biological investigations, encompassing aptamer-related studies. Incorporating fluorescence reagents into aptamers facilitates heightened sensitivity in various imaging and diagnostic endeavors.
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Biotin Modification Aptamer
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Biotin modification holds significance within the realm of aptamer investigation, serving as a pivotal tool for proficiently labeling aptamers with fluorophores in techniques like EMSA or Southern blotting. Additionally, biotin modification enhances the tethering of aptamers to nanospheres or magnetic beads.
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Features of Our Aptamer Modifications Services
Advancing Modifications
Utilizing forefront methodologies to facilitate a range of aptamer modification strategies
Ensuring Consistency
Achieving Stable and Reproducible Aptamer Products
Navigating Expertise
Immediate technical counsel offered by our adept team, drawing on extensive expertise
Stringent QC
Our aptamer modification service incorporates a rigorous QC system to ensure a stable and consistent product output.
Creative Biolabs provides comprehensive services for aptamer modification. For further information, please don't hesitate to contact us.
Reference
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Niederlender, Solène, Jean-Jacques Fontaine, and Grégory Karadjian. "Potential applications of aptamers in veterinary science." Veterinary Research 52.1 (2021): 1-20.
Questions & Answer
A: Altered aptamers can be fabricated through solid-phase synthesis methodologies, wherein distinct positions are targeted for the introduction of chemical alterations or conjugations throughout the synthetic procedure.
A: Creative Biolabs provides an extensive array of aptamer modifications, encompassing chemical modifications, backbone modifications, nucleotide modifications, aptamer labeling, conjugation with functional groups or molecules, and aptamer truncation.
A: The design of modifications to aptamers necessitates meticulous consideration to prevent any compromise to the aptamer’s binding affinity or the introduction of potential toxicity. The choice of modification type and position should be considered in relation to the intended application. What’s more, the timeline for aptamer modification services can vary depending on the specific modifications required and the complexity of the project. Please consult with us for a detailed timeline based on your requirements.
For Research Use Only.
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