The complement receptor-ligand binding assays are valuable in understanding the molecular interactions between complement receptors and their ligands. With advanced platforms and standard procedures, Creative Biolabs provides rapid and customized complement receptor-ligand binding assays to customers worldwide.
Overview of Complement Receptors Ligand
Complement receptors are a group of proteins found on the surface of various immune cells, including leukocytes (such as neutrophils, macrophages, and dendritic cells) and some non-immune cells. These receptors are involved in the recognition and binding of complement components, particularly the activated fragments of the complement system. These complement receptors have diverse functions and contribute to various immune processes, including phagocytosis, clearance of immune complexes, modulation of immune responses, and cell-cell interactions.
Fig. 1 Complement receptors and their functions.1
Introduction of Receptor Ligand Binding Assay
Method
The complement receptor ligand binding assay is a laboratory test used to assess the interaction between complement receptors and their ligands, which can be performed by radioisotope labeling, receptor-ligand enzyme-linked immunosorbent assay (ELISA), Immunoprecipitation, and surface plasmon resonance (SPR).
Analysis
The results provide information about the strength of the interaction between the complement receptor and its ligand, including the binding affinity and kinetics, and aid in characterizing the roles and functions of complement receptors in immune processes.
Workflow of Receptor Ligand Binding Assay & Case Study
Radioisotope labeling
Receptor-ligand ELISA
Fig. 2 Binding of 125I-MBL to sCR1 using radioisotope labeling.2
Fig. 3 Measurement of the interaction between sCR1 and PfRh4 based on ELISA.3
Immunoprecipitation
Surface plasmon resonance (SPR)
Fig. 4 Measurement of the interaction between sCR1 and PfRh4 based on immunoprecipitation.3
Fig. 5 Use of SPR to measure CR1 and PfRh4 binding.3
Applications
Characterizing receptor-ligand interactions
The assay is commonly used to study and characterize the binding interactions between receptors and their specific ligands to help researchers understand the molecular mechanisms underlying cellular signaling, immune responses, and disease processes.
Drug discovery and development
Screening potential therapeutic compounds or small molecules against specific receptors and their ligands, helps the development of targeted drugs that modulate receptor-ligand interactions and potentially treat various diseases, including autoimmune disorders, cancers, and infectious diseases.
Assessing receptor expression and function
The assay can be used to determine the expression levels and functional activity of specific receptors in different cell types or tissues to understand the roles of receptors in cellular processes and provide valuable information about receptor abnormalities or dysregulation in disease conditions.
Biomarker discovery and validation
The assay can aid in the discovery and validation of biomarkers-specific molecules or patterns associated with particular diseases or disease stages. Identifying reliable biomarkers can contribute to early diagnosis, disease prognosis, and monitoring of treatment response.
Featured Services
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Total Complement Activity Test
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C1q-Binding Assays
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Creative Biolabs offers total complement activity test services for our clients. Total complement activity tests include CH50, AH50, and LP50.
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Creative Biolabs offers high-quality C1q-binding assays for the characterization of therapeutic mAbs.
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Learn more
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Learn more
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Overall, the receptor ligand binding assay provides a valuable tool for understanding receptor-ligand interactions, drug discovery, biomarker identification, and diagnostic research. Creative Biolabs is always committed to providing the highest-quality and most appropriate complement receptor-ligand binding assays for the various needs of clients. Please directly contact us for more details.
References
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Holers, V Michael. "Complement and its receptors: new insights into human disease." Annual review of immunology vol. 32 (2014): 433-59.
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Ghiran, I, et al. "Complement receptor 1/CD35 is a receptor for mannan-binding lectin." The Journal of experimental medicine vol. 192,12 (2000): 1797-808.
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Tham, Wai-Hong, et al. "Complement receptor 1 is the host erythrocyte receptor for Plasmodium falciparum PfRh4 invasion ligand." Proceedings of the National Academy of Sciences of the United States of America vol. 107,40 (2010): 17327-32.
Questions & Answer
A: The assay involves immobilizing the complement receptor on a solid surface and then incubating it with the ligand of interest. The binding between the receptor and the ligand is quantified using various detection methods, such as fluorescence, radioactivity, or enzyme-linked assays. The purpose of this assay is to measure and characterize the binding affinity and kinetics of complement receptors and their ligands, providing insights into their molecular interactions.
A: These assays can be used to study the role of complement receptors in immune responses, inflammation, and diseases such as autoimmune disorders. They can also help in the development of therapeutic agents targeting complement receptors. And with appropriate assay miniaturization and automation, complement receptor-ligand binding assays can be adapted for high-throughput screening of large compound libraries to identify potential modulators or inhibitors.
A: Some challenges include the availability and purity of recombinant complement receptors and ligands, optimization of assay conditions, and potential interference from non-specific binding or other factors that may influence the results.
For Research Use Only.
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