Antibodies

Creative Biolabs can offer a series of ready-to-use antibodies targeting different components of the complement system. Take the advantages of good sensitivity and specificity, these high-quality antibodies could be great tools to assist our clients’ research and development project on the complement system.

Overview Target Classical Pathway Target Alternative Pathway Target Lectin Pathway Target Terminal Pathway Quality and Technical FAQs Popular Products Related Services

Antibodies

The complement system is an essential component of innate immunity and consists of more than 30 proteins that orchestrate immune responses through carefully regulated cascades. Antibodies specific for these components have become a powerful tool for mechanistic studies and therapeutic development.

Creative Biolabs is a leader in complement-targeted antibody solutions. Partner with us for high-quality, customized antibody solutions for the complement system.

Classical Pathway Component Antibodies

The classical pathway is a carefully regulated cascade reaction that is initiated upon recognition of the immune complex by the C1 complex. Upon activation, the C1 complex triggers the following sequential cleavage: C4 breaks down into C4a and C4b, which bind covalently to the target surface, C2 is converted into C2a and C2b, forming the C3 convertase, which then cleaves C3, amplifying the cascade and leading to the formation of the C5 convertase, followed by MAC assembly, and ultimately target cell lysis.

Antibodies directed against specific classical pathway components are indispensable tools for studying complement biology. Creative Biolabs provides key antibodies to components of the classical pathway.

Antibodies	Target	Scientific Applications
Anti-C1q Antibodies	C1q is the pattern recognition molecule of the C1 complex, binding to Fc regions of IgG or IgM antibodies in immune complexes.	Antibodies against C1q are used to dissect the initial steps of complement activation. These antibodies help identify the conformational changes upon immune complex binding. Anti-C1q antibodies are instrumental in understanding complement-related inflammatory diseases and exploring C1q inhibition strategies.
Anti-C1r Antibodies	C1r and C1s are serine proteases that activate downstream components of the pathway.	Specific antibodies facilitate the measurement of enzymatic activity during C1 complex activation. Used in mapping the interaction between C1r/C1s and other pathway molecules. These antibodies can help explore potential targets for inhibiting unwanted complement activation.
Anti-C1s Antibodies
Anti-C2 Antibodies	C4 and C2 are substrates cleaved by the activated C1 complex, forming the C3 convertase.	These antibodies help track the cleavage and deposition of C4b and C2a. Investigating the formation and stability of C4b2a convertase complexes. Anti-C4 and anti-C2 antibodies are valuable for understanding how convertase activity can be modulated.
Anti-C4 Antibodies
Anti-C3 Antibodies	C3 and C5 are critical junctures in the complement system, driving amplification and terminal pathway activation.	Anti-C3 antibodies are used to monitor the deposition and amplification of C3b. Anti-C5 antibodies aid in understanding the initiation of the terminal complement cascade. These antibodies are valuable in exploring approaches to block complement overactivation without impeding host immunity.

Customized Antibody Production

As a leader in the development of high-quality antibodies, Creative Biolabs offers a comprehensive portfolio of reagents targeting components of the complement pathway.

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Minimal cross-reactivity with other complement components

High-affinity antibodies enhance the sensitivity of the assay

Well-characterized antibodies that have been validated in a variety of applications

Alternative Pathway Component Antibodies

The alternative pathway is characterized by its spontaneous activation mechanism. Key to this process is the hydrolysis of complement component C3, resulting in the formation of C3(H₂O), which triggers downstream events. The pathway serves as both a primary defense and an amplification loop for other complement activation routes.

Creative Biolabs is a leader in providing high-quality complement antibodies that support advanced studies in alternative pathway.

Anti-C3 Antibodies

Detection and quantification of native C3 and its cleavage products (C3a, C3b, iC3b, and C3d).
Functional assays to monitor complement activation and identify dysregulated C3 activity.

Anti-Factor B Antibodies

Analysis of Factor B binding to C3b and its cleavage by Factor D.
Monitoring of C3 convertase formation and stability.

Anti-Factor D Antibodies

Inhibition studies to block Factor D activity in complement assays.
Functional exploration of serine protease activity in the alternative pathway.

Anti-Properdin Antibodies

Studying the stabilization of C3 convertase in alternative pathway activation.
Functional assays to understand properdin's role in complement amplification.

Anti-Factor H Antibodies

Investigation of regulatory mechanisms governing C3b degradation.
Studies on Factor H polymorphisms and their role in immune regulation.

Anti-Factor I Antibodies

Investigation of regulatory mechanisms governing C3b degradation.
Studies on Factor I polymorphisms and their role in immune regulation.

Lectin Pathway Component Antibodies

The core components of the lectin pathway include Mannose-Binding Lectin (MBL), Ficolins, Collectins, MBL-Associated Serine Proteases (MASP). Antibodies targeting these components have proven instrumental in unraveling their biological roles and interactions.

Anti-MBL Antibodies

IP: MBL antibodies can capture MBL complexes for proteomic studies.
WB: Detecting the expression of MBL in cell lysates and serum.
ELISA: Quantifying MBL levels in serum samples to study its role in inflammation or infection.
Structural Studies: Epitope-specific MBL antibodies aid in mapping carbohydrate recognition domains (CRDs).

Anti-Ficolins Antibodies

ELISA: ELISA-based assays using anti-ficolin antibodies are crucial for measuring their levels in plasma or supernatants.
Flow Cytometry: Detecting ficolin expression on cell surfaces or in secreted forms.
Functional Studies: Antibodies that block ficolin binding can elucidate their role in complement activation.

Anti-MASPs Antibodies

Specific Antibodies: Distinguish between MASP-1 and MASP-2.
Inhibitory Antibodies: Block MASP activity to explore the therapeutic potential of complement-mediated diseases.

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Let us know how we can customize any product to meet your specific project needs.

Monoclonal antibodies
Polyclonal antibodies
Recombinant antibody fragments
Engineered antibodies

Specificity testing
Cross-reactivity analysis
Functional validation
Application-specific testing

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Terminal Pathway Component Antibodies

Activation of the complement pathway culminates in the terminal complement pathway, a tightly regulated series of events that leads to MAC formation. The terminal pathway is mediated by components C5, C6, C7, C8, and C9, which assemble sequentially to cleave the target cell membrane.

Given the complexity and importance of terminal pathways, specific antibodies against their components have become powerful tools for research and therapeutic development. Creative Biolabs is dedicated to providing cutting-edge solutions for complement research and antibody development.

Antibodies	Targeted Role	Applications
Anti-C5 Antibodies	Inhibiting the initiation of the terminal pathway by blocking C5 cleavage.	Investigating the role of C5a and C5b in immune responses. Blocking C5-mediated MAC formation in vitro models of inflammatory and autoimmune diseases. Developing therapeutic inhibitors for diseases like PNH and aHUS.
Anti-C6 Antibodies	Preventing the formation of the C5bC6 complex.	Studying the interaction dynamics of terminal pathway proteins. Monitoring complement activation states in research settings.
Anti-C7 Antibodies	Inhibiting membrane binding of the C5bC6C7 complex.	Identifying regulatory roles of C7 in complement activation. Evaluating the assembly and functionality of the MAC.
Anti-C8 Antibodies	Blocking membrane penetration of the MAC.	Research into MAC-associated cell lysis and immune regulation. Analyzing complement-mediated cytotoxicity in various models.
Anti-C9 Antibodies	Preventing C9 polymerization and pore formation.	Probing the structural and functional dynamics of the MAC. Assessing the contribution of MAC formation to tissue damage in complement-driven disorders.

Quality Control and Technical Considerations

We focus on the scientific and technical considerations of antibodies targeting complement components. Key aspects include antibody form, validation methods, quality control standards, and comprehensive documentation to help our customers select the right antibody.

1

Antibody Formats

Selecting an appropriate antibody format is a critical step in targeting complement components. Each format offers unique advantages depending on the specific application, including monoclonal antibodies (mAbs), polyclonal antibodies, recombinant antibody fragments, engineering modified antibodies.
2

Validation Methods

Thorough validation ensures the reliability of antibodies in non-clinical studies. We offer the following methods: specificity testing (e.g. ELISA, WB, or IHC analysis), cross-reactivity analysis (to minimize false-positive results), functional validation (to assess the ability of an antibody to modulate or neutralize complement activity), and application-specific testing.
3

Manufacturing Standards

We ensure consistent manufacturing processes (standardized protocols for cell line development, culture conditions, and purification techniques), high purity (SDS-PAGE, size exclusion chromatography, and mass spectrometry analyses), stability, and batch-to-batch reproducibility (to ensure consistency of binding activity and functional efficacy).
4

Documentation

Our key documents include COA, validation data, technical specifications, storage and handling guidelines.

Through careful selection of antibody forms, rigorous validation methods, adherence to high manufacturing standards, and comprehensive documentation, researchers can achieve reliable and reproducible results.

Analytical Techniques

Creative Biolabs offers a comprehensive range of advanced analytical techniques including WB analysis, immunoprecipitation, flow cytometry, ELISA development, and more.

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Research Protocolss

Creative Biolabs offers comprehensive and user-friendly research protocols, including sample preparation guidelines, experimental design considerations, controls and standardization, data analysis methods, etc.

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Get a Free Brochure

Creative Biolabs offers a range of high-quality products for complement activity and function assays. View our product list to find your interested products.

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Complement Function Analysis Protocols

We briefly summarize the complement-related assay protocols. Find complement-related assay protocols by clicking the following items.

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FAQs

Are your complement system antibodies suitable for use in multiplex assays, and how can I avoid cross-reactivity issues?

Our antibodies are specifically designed and validated for multiplex compatibility. We carefully select epitopes and antibody isotypes to minimize cross-reactivity when using multiple antibodies simultaneously. We provide detailed recommendations for antibody combinations and can suggest optimal dilutions for multiplex experiments. Our technical support team can help you design your multiplex panels and troubleshoot any potential cross-reactivity issues. We also offer specific blocking reagents if needed.

Can you provide custom formulations or bulk quantities of complement system antibodies for large-scale studies?

Yes, we offer customization options for our complement system antibodies. We can adjust buffer compositions, concentrations, and volumes according to your requirements. For bulk orders, we provide competitive pricing and can establish a customized production schedule to ensure consistent supply for your large-scale studies. We also offer custom conjugation services and can develop specific formulations optimized for your particular application or storage conditions.

What species reactivity do your antibodies cover, and how thoroughly is this validated?

Our complement system antibodies are validated for multiple species including human, mouse, rat, and other commonly used research models. Each antibody undergoes extensive cross-species reactivity testing using various techniques. We provide detailed species reactivity data in our datasheets, including sequence homology analysis and experimental validation results. For novel applications or untested species, we can perform custom validation studies.

Are your antibodies suitable for multiplexing experiments with other markers?

Many of our complement system antibodies are compatible with multiplexing experiments. We offer antibodies conjugated to various fluorophores and provide detailed spectral information to help plan multi-color experiments. Our technical team can assist in designing panels to minimize spectral overlap and recommend optimal antibody combinations.

Can these antibodies be used for blocking complement activation in functional studies?

While our antibodies are primarily validated for detection applications, select antibodies in our catalog have been tested for their blocking capabilities. These functional antibodies are specifically identified in our product documentation.