Introduction Workflow DeliverablesCase Study Why Choose us?Published Data FAQ Related Services Featured Products
Introduction
Dysregulation of the complement system, an essential component of innate immunity, is a key driver of various inflammatory diseases, particularly thrombotic microangiopathies (TMAs) that affect the kidney. The terminal product of this cascade, the Membrane Attack Complex (C5b-9), is a validated biomarker for disease activity. Creative Biolabs provides high-quality C5b-9 deposition assay to clients all over the world.
C5b-9 Deposition Assay Workflow
Samples Required serum, plasma, cell suspensions, drugs or inhibitors need to test. You can contact us for detailed information.
Key Steps Involved
01
Sample preparation, in which Creative Biolabs receives and handles samples based on validated protocols, ensuring maximum sample integrity and assay compatibility.
02
Regulated complement activation, attained via meticulously defined incubation parameters that promote C5b-9 deposition.
03
C5b-9 Deposition: High-sensitivity C5b-9 detection, employing specific antibodies and advanced methodologies, such as ELISA or flow cytometry, for accurate quantification.
04
Data Analysis & Interpretation: The raw quantitative data is statistically analyzed and compared against internal control samples to determine the degree of complement overactivation.
05
Final Deliverables: Upon completion of the service, clients receive a detailed project report. This includes a clear executive summary of findings, a description of the experimental methodology, and statistical analysis summaries & raw data files.
Estimated Timeframe The typical timeframe for this service ranges from 4 to 6 weeks, depending on the number of samples submitted and the complexity of the requested analysis.
Fig.1 C5b-9 deposition assay by ELISA detection.
Deliverables
-
Quantitative data on serum-induced C5b-9 deposition (e.g., fluorescence intensity, deposition area).
-
A detailed report including a summary of findings, methodology, and statistical analysis.
-
Raw data files from image analysis for independent review.
Case Study
Fig.2 C5b-9 deposition assay test in the normal human serum.
Fig.2
Creative Biolabs carried out C5b-9 deposition assays on samples provided by clients. The accompanying figure demonstrates a typical C5b-9 deposition assay. The findings revealed that the sample inhibited C5b-9 deposition in normal human serum. This assay was conducted using 10 serial dilutions, with each dilution assessed in duplicate. Additional customization of the experimental plan can be arranged according to specific needs.
Why Choose Us?
At Creative Biolabs, we offer a comprehensive, one-stop solution for all your complement-related research needs. Rather than piecing together different services from multiple vendors, you can rely on our integrated approach to provide a seamless and efficient experience.
Integrated Workflow:
Our C5b-9 Deposition Assay is part of a broader suite of services that can be tailored to your project. From foundational complement pathway functional assays to the development of highly specific antibodies, we provide end-to-end support. This integrated workflow ensures consistency, accuracy, and efficiency across all stages of your research.
Expert Guidance:
Our team of experienced scientists will collaborate with you to design the most effective experimental plan. We provide expert advice on sample handling, data interpretation, and next steps, helping you navigate complex biological questions with confidence.
Accelerated Timelines:
Our streamlined process and on-site expertise allow us to deliver results faster than a fragmented approach. This can significantly accelerate your project timelines, helping you move from discovery to clinical application more quickly.
Unparalleled Quality:
We maintain the highest standards of quality control and scientific rigor across all our services. By keeping the entire process under one roof, we guarantee the integrity and reliability of your data.
Published Data
Our methodology is grounded in published, peer-reviewed science that highlights the value of functional complement assays. A comprehensive review examines the critical role of C5b-9 as a biomarker and therapeutic target. The article highlights that while traditional methods like ELISA measure soluble C5b-9, more precise methods, such as immunohistochemistry and flow cytometry, are crucial for visualizing and quantifying C5b-9 deposition in tissues and on cells. This approach provides a superior method for both diagnosis and monitoring of therapeutic interventions.
Fig.3 C5b-9 deposition in normal and diseased human kidneys.1
Frequently Asked Questions
A: The C5b-9 Deposition Assay is a functional test that measures the pathogenic potential of serum to activate complement on a cell surface, while a simple sC5b-9 ELISA quantifies soluble complexes in the blood. Our assay provides a more direct measure of disease activity by mimicking the in vivo environment, whereas the ELISA measures systemic activation, which may not reflect localized tissue damage.
A: This service is ideal for biopharmaceutical companies developing complement inhibitors, clinical researchers studying the mechanisms of complement-mediated disorders.
A: Yes, our assay has been shown to detect increased complement activation in asymptomatic carriers of pathogenic gene mutations and during subclinical episodes, making it a powerful tool for early intervention studies.
A: Proper sample collection and handling are crucial. To ensure accurate results, serum or plasma samples must be collected and frozen rapidly to minimize in vitro complement activation. Our team will provide detailed instructions to ensure your samples are processed correctly.
A: Absolutely. By analyzing paired samples from pre- and post-treatment time points, our assay provides a direct measure of how effectively your therapeutic candidate is blocking the formation of the terminal complement complex. This is invaluable for establishing proof of concept and defining optimal dosing.
Related Services
Creative Biolabs also offers a range of Complement Test Services, Which can further support your research in the complement system.
Featured Products
The Creative Biolabs C5b-9 Deposition Assay Service provides a critical, functional diagnostic tool for advancing research and clinical development in complement-mediated kidney diseases. By moving beyond traditional soluble biomarkers, we offer a service that is directly relevant to patient outcomes and therapeutic success. Contact our team to learn how our expertise and technology can provide the data you need to drive your project forward.
Reference
-
Koopman, Jacob JE, et al. "Deposition of the membrane attack complex in healthy and diseased human kidneys." Frontiers in immunology 11 (2021): 599974. Distributed under Open Access license CC BY 4.0, without modification. DOI: https://doi.org/10.3389/fimmu.2025.1589584
For Research Use Only.
Related Sections:
