Introduction Workflow DeliverablesWhy Choose us?Published Data FAQ Related Services Featured Products
Introduction
The Complement Fixation Test is a classical serological method for detecting specific antibodies or antigens. This assay is based on the principle that Ag-Ab complexes fix and consume serum complement. Historically pivotal for diagnosing diseases such as syphilis and Q fever, it relies on a two-stage reaction system and subjective hemolysis readout.
Complement Fixation Assay Workflow
Samples Required
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Serum: The most common sample type for antibody detection.
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Plasma: Ideal for studies where complement activation needs to be assessed without the confounding effects of serum-derived components.
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Cerebrospinal Fluid (CSF): Applicable for specialized neurological or infectious disease studies.
Key Steps Involved
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Sample Preparation & Pre-screening: Required Starting Materials: Client-provided serum or plasma samples, along with a detailed sample manifest and known target antigen information.
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Antigen/Antibody Titration: Our experts carefully titrate the provided antigen and/or serum against a standardized source of complement to determine the optimal working dilutions. This critical step ensures accurate and reproducible results.
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Complement Fixation & Incubation: The test is performed in the primary fixation stage, where patient samples are mixed with the target antigen and complement. The plate is incubated at a controlled temperature for a specified duration, allowing any existing Ag-Ab complexes to form and consume the complement.
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Indicator System Integration: Our service employs specific antibodies and advanced methodologies, such as ELISA or flow cytometry, for detecting complement activation products.
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Data Acquisition & Analysis: The plate is read using a high-sensitivity spectrophotometer, fluorometer, or flow cytometer. The data is then analyzed to determine the level of complement fixation, which directly correlates to the titer of the specific antibody present.
Estimated Timeframe The typical timeframe for this service ranges from 2 to 4 weeks, depending on the number of samples and the complexity of the target antigen and antibody. Rush services are available upon request.
Deliverables
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Processed datasets (e.g., Quantitative data) in formats.
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A description of the complete experimental methodology and protocol.
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Statistical analysis summary of the results, including data visualizations and raw data files.
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A final report detailing the conclusion and interpretation of the data.
Why Choose Us?
Choosing Creative Biolabs means partnering with a leader in advanced immunoassay services.
Comprehensive, One-Stop Solution:
We handle the entire process from sample receiving to final report, eliminating the need for complex in-house assays.
Expertise in Labile Reagents:
Our team has specialized expertise in managing sensitive reagents to ensure assay integrity.
Quantitative and Objective Readout:
We use advanced methodologies for a quantifiable readout, moving beyond the subjective interpretation of traditional methods.
Accurate and Reproducible Results:
Our meticulous process guarantees high-quality, reliable data for your research.
Published Data
In a recent study published, researchers investigated the antibody targets for complement fixation against the Plasmodium falciparum circumsporozoite protein (CSP) in malaria immunity. This research highlights the shift from historical methods to modern, quantitative immunoassays to understand complex immune responses, providing valuable insights for future vaccine development.
Fig.1 Complement-fixing activity of rabbit anti-CSP IgG across various CSP regions.1
Frequently Asked Questions
A: We can work with a variety of samples, including serum, plasma, and cerebrospinal fluid. We also provide guidance on proper sample collection and handling to ensure the integrity of your samples and the reliability of your results.
A: Our assay employs rigorous controls and is optimized for your specific antigen and antibody pair. We perform careful titrations and pre-screening to minimize non-specific binding and interference from anti-complementary factors, ensuring the data we provide is reliable.
A: The turnaround time is typically 2-4 weeks, depending on the project's scope. Our experienced team works efficiently to process your samples and deliver high-quality data as quickly as possible. We’re happy to discuss your specific timelines and see how we can accommodate them.
A: Absolutely. Our assay is a powerful tool for screening and characterizing therapeutic antibodies that rely on complement-dependent cytotoxicity (CDC) mechanisms. The quantitative data we provide is essential for guiding your lead selection and moving your project forward.
Related Services
Creative Biolabs also offers a range of Complement Test Services, Which can further support your research in the complement system.
Featured Products
Creative Biolabs' Complement Fixation Assay Service offers a modern, efficient, and reliable solution for researchers seeking to analyze complement-mediated immune responses. By leveraging objective, quantitative methodologies, we overcome the limitations of the classical Complement Fixation Test, providing a service that is essential for both foundational research and therapeutic development. For a detailed discussion on your project needs or to learn more about our Complement Fixation Assay Service, please contact our expert team.
Reference
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Kurtovic, Liriye, et al. "Antibody targets and properties for complement-fixation against the circumsporozoite protein in malaria immunity." Frontiers in immunology 12 (2021): 775659. Distributed under Open Access license CC BY 4.0, without modification. DOI: https://doi.org/10.3389/fimmu.2021.775659
For Research Use Only.
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