Creative Biolabs offers high-quality cell-based complement activity assays to help your research
and drug development projects. Our services represent a comprehensive solution for researchers seeking to understand
and manipulate the complex dynamics of the complement system.
Overview Featured Services Applications Key Features Published Data Related Services
Overview
The complement system is an integral part of the immunity response and plays a vital role in defense against
pathogens and immune regulation. Cell-based complement activity assays enable the direct assessment of complement
activation and its consequences on target cells, mimicking in vivo conditions more closely than traditional
biochemical assays. Therefore, cell-based complement activity assays have gradually emerged as a powerful tool to
study the complex interactions between complement components and their target cells, providing insights into
physiological and pathological processes.
Fig. 1 Complement activation
pathways.1,3
Featured Services
At Creative Biolabs, we provide seamless end-to-end cell-based complement activity assay services from assay design
to data analysis. Our experienced scientists rely on advanced methods and well-established procedures to ensure your
complement activity assay project runs smoothly.
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Indirect Immunofluorescence Assay (IFA Assay)
The cell-based complement activity assay, specifically the IFA assay, is a powerful and versatile technique utilized
in immunology and biomedical research to evaluate the functional activity of the complement system. Following are
simple protocols of the IFA assay.
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Cell-Enzyme-Linked Immunosorbent (ELISA) Assay
Cell-ELISA is a sophisticated technique employed in biomedical research and diagnostics to evaluate the activation
and functionality of the complement system within a cellular context. This assay marries the principles of
traditional ELISA with the unique ability to monitor complement-mediated events directly on or near cell surfaces,
providing a more physiologically relevant assessment compared to assays performed in solution.
Applications
Disease Pathogenesis
Illuminated the role of complement in autoimmune diseases, infectious diseases, and inflammatory conditions.
Therapeutic Development
Evaluating the efficacy of complement inhibitors or activators in modulating cellular responses, support the
discovery and development of novel therapeutic strategies.
Drug Safety Testing
Assessing the potential for drugs to activate or inhibit the complement system is crucial for predicting
adverse immune reactions and optimizing drug safety profiles.
Why Choose Us?
Expertise & Experience
Our team of highly skilled scientists boasts extensive experience in immunology and cell-based assays, ensuring the
highest quality results.
Customizable Solutions
We tailor our protocols to your specific research needs, adjusting antibody concentrations, and incubation times, or
even incorporating additional controls as required.
Rigorous Quality Control
Every step is rigorously validated using industry-standard practices, ensuring reproducibility and reliability of
your data.
Comprehensive Reporting
Detailed experimental reports, including raw data, analysis, and interpretation, are provided to empower your
research insights.
Published Data
Fig.2 Osteosarcoma cell-based complement system activitation.2,3
Recent research indicates that complement system activation plays a crucial role in tumor progression. Human bone osteosarcoma epithelial cells (U2-OS) have been shown to trigger the alternative complement pathway when exposed to pooled normal human serum. A cell-based ELISA method was developed to measure complement activation on the surface of eukaryotic cells. Verification of complement system activation by U2-OS cells involved analyzing MAC and C3b deposition using immunofluorescence and flow cytometry. PCR tests confirmed the absence of mycoplasma contamination. Post-complement activation, cell viability assessments revealed U2-OS cells as potential models for studying complement activation with sublytic MAC levels. MAC deposition on osteosarcoma tissues corroborates complement activation in these cancers. Tissue microarray experiments demonstrated evident MAC staining on tumor cells, suggesting that targeting the complement system could be a promising therapeutic strategy for cancer.
Related Services
Contact us today to advance your complement research endeavors.
References
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Ng, Nicole, and Charles A. Powell. "Targeting the complement cascade in the pathophysiology of COVID-19 disease." Journal of Clinical Medicine 10.10 (2021): 2188.
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Jeon, Hyungtaek, et al. "Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors." Scientific reports 8.1 (2018): 5415.
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Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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