Anti-VEGF165 Aptamer(Cat#: CTApt-M-1974)
This product is an aptamer which binds to the Vascular Endothelial Growth Factor with an affinity of <1 nM.
Specific Inquiry 
Specifications
| Target Category |
Protein |
| Target |
Vascular Endothelial Growth Factor |
| Alternative Names |
Vascular Endothelial Growth Factor A; VPF; VEGF-A; VEGF; Vascular Permeability Factor; Vascular Endothelial Growth Factor A121; Vascular Endothelial Growth Factor A165; Vascular Endothelial Growth Factor; MVCD1 |
| Gene ID |
7422 |
| UniProt ID |
P15692 |
| Target Overview |
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. |
| Chemical Modification |
DNA |
| Length |
60-80 nt |
| Affinity (Kd) |
<1 nM |
| Binding Conditions/Buffer |
SPR Buffer A: 10 mM phosphate buffered saline; 138 mM NaCl; 2.7 mM KCL; pH 7.4 with 0.005% Tween 20. |
| GC Content |
0.6154 |
| Molecular Weight |
7981.22 g/mole |
| Extinction Coefficient |
249100 L/(mole·cm) |
| nmoles/OD260 |
4.01 |
For Research Use Only. Not for Diagnostic or Therapeutic Applications.
