Functional analysis and characterization that reflect the drug’s mechanism of action (MOA) are required in the process of antibody drug discovery, to ensure efficacy and safety. Currently, Creative Biolabs offers a wide range of service portfolio to support new innovator or biosimilar therapeutic antibody development, including functional cell-based assays that allow the evaluation of antibody-dependent cell-mediated phagocytosis (ADCP).
Monoclonal antibodies (mAbs) are a booming group of biotherapeutics which have been successfully utilized in clinical practice for the treatment of cancer and autoimmune diseases. The targeted killing action of immune effector cells to the diseased cell is one of the vital MOAs for naked therapeutic antibodies, which is realized by the interaction of the Fc domain of the antibody with the complement component C1q or Fcγ receptors. However, many of these antibodies have failed in the clinic due to poor efficacy.
In order to develop more potent therapeutic mAbs, their biological effector functions should be considered to be increased through the enhancement or reduction in their ability to mediate cellular cytotoxicity functions such as ADCP. ADCP, briefly speaking, is the mechanism by which antibody activates the macrophages by binding to the FcγRs, thereby inducing phagocytosis and lysosomal degradation of tumor cells (Fig.1).
Fig.1 Antibody-dependent cellular phagocytosis process.1
For the classical primary ADCP assay, monocytes derived from PBMC of healthy donors by negative selection are utilized as effector cells after differentiation to M2 macrophages. Our scientists have developed robust and highly accurate primary ADCP assay formats reflecting activity via CD32 and CD64, using genotyped donors. Our services are characterized by:
Based on our versatile menu of assays, flexible technology platforms, and comprehensive scientific expertise, Creative Biolabs is able to provide a wide range of bioassay services to evaluate the interaction of antibodies with Fc receptors to modify specific immune cell-mediated interactions depending on the specific research requirements of our clients.
Now, we provide two distinct well-established assay formats for ADCP evaluating:
In addition to ADCP assays, Creative Biolabs also offers the following services regarding Fc-mediated Effector Functional Assays:
If you have any special needs in primary ADCP assays or be interested in learning more about Creative Biolabs’ ADCP assay services, please contact us for more information.
Reference
For Research Use Only.