Harness the power of covalent chemistry to build superior molecular constructs. At Creative Biolabs, we translate your complex biological questions into functional solutions, accelerating your journey from discovery to market.
What is Bioconjugation?
Bioconjugation is the strategic chemical process of forming a stable, covalent link between two molecules, where at least one is a biomolecule such as a protein, antibody, or nucleic acid. This powerful technique creates hybrid molecules, or 'conjugates,' that possess the unique functionalities of each component. By precisely combining the targeting specificity of an antibody with the therapeutic potency of a small molecule drug, for example, we can create highly effective and targeted therapies like Antibody-Drug Conjugates (ADCs).
Choosing bioconjugation as a strategy is choosing precision and innovation. It allows for the development of novel diagnostic tools, advanced imaging agents, and next-generation therapeutics with improved efficacy, reduced side effects, and enhanced.
Comprehensive Bioconjugation Services
Bioconjugation Techniques
The success of a bioconjugation project hinges on selecting the right chemical strategy. Our scientists are proficient in a comprehensive array of modern and classical conjugation techniques, ensuring we apply the most appropriate method based on your molecules of interest, desired stability, and final application. We optimize every reaction to maximize yield and purity while preserving the biological activity of the components.
Our technical capabilities are extensive and include:
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Chemical Conjugation: Employing classical chemistries that target specific reactive functional groups on biomolecules.
- EDC/NHS Coupling
- Maleimide Chemistry
- Aldehyde/Ketone Chemistry
- Disulfide Bond Formation
- Enzymatic Conjugation: Using highly specific enzymes to catalyze the formation of covalent bonds at defined sites.
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Bioorthogonal Chemistry: Leveraging a suite of highly efficient and biocompatible reactions that proceed with minimal side products.
- Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)
- Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)
- Tetrazine Ligation
- Staudinger Ligation
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Affinity and Other Advanced Methods: Utilizing strong molecular interactions or novel biological techniques for conjugation.
- Biotin-Streptavidin Interaction
- Metal Chelation
- Hydrophobic Insertion
Applications of Bioconjugation
Creating high-sensitivity probes and reagents for ELISA, Western Blot, flow cytometry, and IHC.
Engineering targeted drug delivery systems to increase therapeutic index and reduce off-target toxicity.
Developing contrast agents and fluorescent probes for real-time molecular imaging in living organisms.
Immobilizing enzymes on solid supports to create robust, reusable biocatalysts for industrial processes.
Using cross-linking agents to capture and identify protein-protein or protein-nucleic acid interactions.
Linking carbohydrate antigens to carrier proteins to elicit a robust immune response.
Creating novel biopharmaceuticals, including antibody-drug conjugates, PEGylated proteins, and peptide-drug conjugates.
Our Streamlined Workflow
Why Creative Biolabs is Your Ideal Partner
Unmatched Expertise
Our PhD-level scientists possess decades of collective experience in synthetic chemistry and molecular biology. We are not just service providers; we are your collaborative partners, providing insightful solutions to complex challenges.
State-of-the-Art Facilities
Our labs are equipped with advanced instrumentation for synthesis, purification (HPLC), and characterization (LC-MS, SDS-PAGE), ensuring the highest quality and purity for your conjugates.
Customized Solutions
We understand that no two projects are alike. We pride ourselves on our flexibility and ability to develop fully customized conjugation strategies tailored to your unique targets, payloads, and research goals.
Commitment to Quality
Rigorous in-process and final quality control are at the core of everything we do. We provide comprehensive certificates of analysis to ensure your conjugate meets all required specifications with full transparency.
Ready to Advance Your Research?
Let our experts help you design and execute the perfect bioconjugation strategy. Get in touch today to schedule your free project evaluation and receive a detailed proposal.
Frequently Asked Questions
Q: What information is needed to get a quote?
A: To provide an accurate quote and strategy, we typically need to know the identity and quantity of the molecules to be conjugated, any specific functional groups to be used, the desired linker chemistry (if known), and the final application of the conjugate.
Q: How long does a project usually take to complete?
A: Turnaround time varies depending on project complexity, from 2-4 weeks for standard conjugations to several weeks for multi-step custom projects. A detailed timeline will be provided in your project proposal.
Q: How do you handle client confidentiality?
A: We operate under strict confidentiality agreements (CDA/NDA) to protect your sensitive project information. We are dedicated to making sure your confidential data is completely secure.
Q: What quality control (QC) data will I receive?
A: Every project includes a comprehensive Certificate of Analysis (CoA) with relevant QC data, which can include HPLC for purity, Mass Spectrometry for identity confirmation. For ADCs, we provide precise Drug-to-Antibody Ratio (DAR) analysis.
Q: Can you accommodate both small-scale and large-scale projects?
A: Yes. We are equipped to handle projects ranging from small-scale discovery and proof-of-concept studies (milligram scale) to larger-scale production runs required for preclinical development.
Related Resources
Documentation
Clear brochures and infographics to accelerate understanding and streamline experimental design in bioconjugation research.
Tech Note: Bioorthogonal Chemistry
A technical overview of how bioorthogonal reactions are revolutionizing bioconjugation.
Protocols
Access our library of step-by-step protocols for a variety of common bioconjugation chemistries and applications.