This product is an aptamer which binds to the Caffeine with an affinity of <1 mM.
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| Target Category | Protein |
| Target | Caffeine |
| Chemical Modification | RNA |
| Length | 80-100 nt |
| Affinity (Kd) | <1 mM |
| Binding Conditions/Buffer | Selection Buffer (500 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 10 mM sodium phosphate, 90 mM HEPES (pH 7.5)) Assay Buffer (180mM HEPES (pH 7.0?ñ8.0), 300 mM NaCl, 20 or 50 mM MgCl2, 2 mM EDTA, 20 mM Na phosphate), r.t. |
| GC Content | 51.52 |
| Molecular Weight | 31866.13 g/mole |
| Extinction Coefficient | 976100 L/(mole·cm) |
| nmoles/OD260 | 1.02 |
Exceptional specificity and affinity towards caffeineExcellentThe aptamer was easy to use and delivered consistent results. Highly recommended!
It significantly improved the accuracy of my assaysExcellentI successfully utilized the aptamer in my experiments and achieved reproducible and reliable results.
An excellent tool in my drug discovery projectExcellentIt displayed remarkable affinity towards Caffeine and demonstrated minimal cross-reactivity with other compounds.