Anti-Creatinine Aptamer(Cat#: CTApt-865)
This product is an aptamer which binds to the Creatinine with an affinity of <50 µM.
Specific Inquiry 
Summary
Published Data
Customer Reviews
Related Products & Services
Resources
Specifications
| Target Category |
Small Organic |
| Target |
Creatinine |
| Target Overview |
Creatinine is a waste product that comes from the normal wear and tear on muscles of the body. Everyone has creatinine in their bloodstream. |
| Chemical Modification |
DNA |
| Length |
40-60 nt |
| Affinity (Kd) |
<50 µM |
| Binding Conditions/Buffer |
20 mM HEPES, 1 M NaCl, 10 mM MgCl2, 5 mM KCl, pH 7.5. |
| GC Content |
0.42 |
| Molecular Weight |
16514.8 |
| nmoles/OD260 |
1.65 |
Background
|
Creatinine, a metabolic byproduct of muscle metabolism, serves as a vital indicator of renal function due to its consistent production and clearance primarily through glomerular filtration. However, conventional methods for creatinine detection often lack specificity and sensitivity, limiting their efficacy in certain experimental settings. In response, anti-creatinine aptamers offer a promising alternative. These single-stranded DNA or RNA molecules bind to creatinine specifically and with high affinity. This aptamer is primarily used as a research tool for studying renal dysfunctions or for developing creatinine detection assays. |
Published Data
Fig. 1 Performance characterization of biosensors with in situ encapsulation of anti-creatinine aptamers and AuNPs in ZIF-8 substrates.
This study used gold nanoparticles and anti-creatinine aptamers encapsulated with ZIF-8 compounds to develop novel hybrid capture probe materials as creatinine biosensors. Extensive material analysis was performed using physical and chemical characterization methods. The electrochemical behavior of the sensors was analyzed using EIS and CA, as well as as as a function of the binding of creatinine expressed in test urine samples to the ZIF-8 aptamer capture probes.
Ganguly, Antra, Anirban Paul, and Shalini Prasad. "Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing." Chemosensors 11.11 (2023): 557.
Fig. 2 ITC characterization of the creatinine aptamer.
Researchers identified and characterized a selective anti-creatinine aptamer for creatinine detection. They performed ITC experiments and showed that the aptamer binds to creatinine with a calculated Kd value of 19 µM. Creatinine binding to the aptamer was found to be driven by a negative enthalpy change, suggesting that it is an exothermic process.
Lyalina, Tatiana. "Novel electrochemical aptamer-based sensing mechanism inspired by selection strategies." (2023).
Customer Review
Successful ELONA of creatinine with aptamers
Excellent
I am interested in ELONA for the determination of creatinine. Then I purchased the anti-creatinine aptamer and successfully applied it to the assay.
The ideal target molecule for in vivo imaging
Excellent
The small size of the aptamer led to its modification into an aptamer-based probe. This helped me to observe the distribution and levels of creatinine in vivo during the progression of kidney disease. And the end result was good.
Measure creatinine levels with anti-creatinine aptamer
Excellent
My experience with this aptamer is that it is selective and stable, and easier to control and cheaper than other reagents.
The purity and effectiveness of this product is truly commendable
Excellent
This anti-creatinine aptamer is a dream come true for a Bio-lab like ours. It worked as expected for our research. We also appreciate the technical guidance provided by the seller which made our work a lot easier.
The product did perform well and delivered satisfactory results
Excellent
As a professor in biochemical research, I can vouch for the anti-creatinine aptamer's high quality. It's become an invaluable tool in our lab, and its consistent performance has made our testing far more accurate and efficient.
For Research Use Only. Not for Diagnostic or Therapeutic Applications.
