We perform splenocyte fusion on one or more animals. We select and subclone the strongly positive clones from the parental cultures by 2 rounds of limiting dilution cloning. Stable clones will be expanded into 24-well plates and then into T-flasks for further characterization. At no additional cost, client could select one clone for in vitro production of approximately 1L of supernatant. Supernatants will be purified using Protein A/G column and pure antibodies will be deliverable. We also offer a full range of contract manufacturing services, including in vitro Antibody and Protein Production in bioreactors and large-scale Antibody Purification.
Our high affinity hamster antibodies is suitable for common research applications, including Chromatin Immunoprecipitation, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot.