Quantitative Tissue Distribution Studies

As a pioneer company in drug discovery process, Creative Biolabs has gained a wealth of experience in providing PK/PD services for global pharmaceutical company and independent researcher. Leveraging our extensive experience and advanced technology, now we can perform quantitative tissue distribution studies to help our worldwide customers with the evaluation of their drug candidates. Our professional staffs are responsible for study designs and data analysis according to your requirements.

Quantitative Tissue Distribution Studies

Tissue distribution study plays a vital role in drug development programs as it can provide important information on the distribution of test compound into tissues of the animals selected for preclinical assessment. Theoretically, each part of the body can receive different doses of the drug and remain the drug in the different amount of time. Traditionally, investigations are performed by conventional methods, such as tissue dissection, total radioactivity determination, and LC-MS-MS/QPCR. However, the appearance of quantitative whole-body autoradiography (QWBA) has reformed the drug distribution research by utilizing phosphor imaging technology and offering high-resolution images.

Quantitative Whole-body Autoradiography (QWBA)

Quantitative whole-body autoradiography (QWBA) is a widely used method utilizing phosphor imaging technology to determine the distribution and concentrations of radiolabeled test compounds in laboratory animals. The most significant advantage of QWBA is that drug concentrations reflected by this technique can be maintained at the time point of euthanasia which cannot be obtained by any other techniques at present. Importantly, QWBA can provide information on tissue PKs, penetration, accumulation and retention.

Application of QWBA

• Target organ/tissue validation
• Tissue distribution
• Placental transfer
• Penetration of blood-brain barrier
• Dermal/ungual penetration
• Human dosimetry calculations
• Tumor penetration

Quantitative Tissue Distribution Study Services Provided by Creative Biolabs

Fig.1 Anatomical corroboration of the microPET signal by digital whole-body autoradiography. (Dubey, et al. 2003)

Creative Biolabs is a professional provider of quantitative tissue distribution study services. In addition to QWBA study services, we also provide autoradiography and micro-autoradiography services utilizing autoradiographic technique to provide high-resolution images of the spatial distribution and match tissue concentrations of drug-related radioactivity throughout the body of laboratory animals. Moreover, we also provide customized services with numerous isotopes labeled to meet every client’s requirements, such as ¹⁴C, ¹²⁵I, ¹⁵³Gd, ³⁵S, ⁵⁹Fe, ³²P, and ⁸⁹Sr.

Creative Biolabs is committed to providing high-quality in vivo PK services for global clients in the most cost-effective way. Except for quantitative tissue distribution study services, we also offer other In vivo ADME services, including:

• Mass Balance Studies
• Metabolite Identification and Profiling
• Excretion Balance

In addition to the services of in vivo ADME, you might be also interested in other in vivo PK studies listed below:

Our staffs will strictly observe the high-quality standards in compliance with GLP regulation or non-GLP regulation to ensure the reliability of data. For more details and information, please feel free to contact us.

Reference

1. Dubey, et al. "Quantitative imaging of the T cell antitumor response by positron-emission tomography." Proceedings of the National Academy of Sciences 100.3 (2003): 1232-1237.

For Research Use Only.