Immunogen Design Service

Creative Biolabs has thorough experience in the design of immunogen based on synthetic peptide and recombinant protein expression. Our long experience and expertise in generating antibodies has shown us the value of drawing from a repertoire of antigen design options, immunization schedule, host species, and screening strategies to build successful antibody discovery projects. Final epitope mapping service, bioinformatics analysis, and computational modeling are also available for clients.

Peptide-based Immunogen Design

Using synthetic peptides as immunogen is the simplest way to produce antibodies against difficult-to-purify proteins or putative protein sequences derived from DNA sequence information. As the epitope is well defined, this method is also useful to target a specific isoform of a given protein, or a specific region such as its active site or an extracellular domain. Peptide immunogen must be carefully designed since they must perfectly mimic the protein region from which their sequence originates. Otherwise, the antibodies nicely recognize the peptides will not recognize the protein.

Many factors should be considered when the immunogen is designed, such as length, molecular weight, secondary structure, purity, hydrophobicity, amino acids. Based on the following four major criteria, Creative Biolabs provides a full range of immunogen design services for hybridoma development. We strongly suggest injecting 2 or 3 distinct peptides to increase the stimulation of the immune system and to target several epitopes of the protein.

Fig.1 Some major factors in immunogen design.

Protein-based Immunogen Design

Proteins are widely used to develop monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) due to their high number of distinct epitopes, leading to very high immunogenicity. Recombinant or native proteins are good choice to develop antibodies which are expected to work in a wide range of applications.

Sometimes, the presence of conserved patterns within some protein families might be a concern in terms of cross-reactivity, and the presence of tags within recombinant proteins may lead to the production of anti-tag antibodies. Moreover, some proteins may be difficult to purify or require specific purification processes, thus increasing production costs. Our customized bioinformatics studies may be used to help select the best immunogen considering critical features:

Antigen drift
Homology with non-relevant proteins
B epitopes prediction
Protein structure analysis

Protein antigens can be the following format.

Inclusion bodies or soluble protein expressed by different expression system
Inactivated virus, bacteria, inactivated yeast
Cell or tissue extracts

Anti-Post-translational Modifications Antibody

Post-translational modifications (PTMs) are chemical modifications that play an important role in functional proteomics. Our scientists are specialized in both the PTM and the peptide synthesis fields. Using this knowledge, Creative Biolabs provides a high-quality service to develop specific antibodies against PTMs using peptides modified in vitro. Peptides bearing various PTMs can be produced, including phosphorylation, methylation, acetylation, and many others.

Creative Biolabs takes advantage of cutting-edge antigen design portfolio to make your project a success. Reach out to our dedicated scientists to discuss how we can assist you with your hybridoma production request today!

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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