E. coli based Virus-Like Particle (VLP) Production Service

Creative Biolabs has established a global-leading virus-like particle (VLP) manufacturing platform using E. coli cell system. Our team has years of experience in VLP construction based on our well-run bacterial expression system. Highly efficient and comprehensive services have earned us a great reputation from numerous clients all over the world.

Introduction of VLP Production in E. coli Cell Expression System

The most popular bacterial host cell for VLP production is Escherichia coli. An expression system based on E. coli has many benefits, such as low cost of production, fast cell growth, high protein expression level, and easy scaling-up. However, because of a number of issues, including incomplete disulfide bond formation, lack of PTM system, and issues with protein solubility, E. coli platforms are not suitable for producing enveloped VLPs. On the other hand, E. coli expression systems are appropriate for producing non-enveloped VLPs with one or two viral structural proteins. Many VLP vaccines against infectious and non-infectious disorders have entered clinical trials. These vaccines were produced using E. coli expression systems. Apart from E. coli, certain additional bacterial species have also been shown to successfully generate VLP.

VLPs production in E.coli expression system. (Nooraei, et al. 2021) Fig.1 E. coli expression systems are appropriate for producing non-enveloped VLPs.¹

Strategies of VLP Production in E. coli Expression System

The proper selection of the expression systems plays a vital role in VLP production. The E. coli cell system is one of the most commonly applied platforms that can satisfy many research and industrial requirements. However, recombinant proteins of eukaryotic hosts are often insoluble in the E. coli cell systems. Creative Biolabs has summed up and utilized a series of solutions to conquer this obstacle:

Lower culture temperature, a very usual way to stimulate the formation of soluble VLPs in E. coli cells.
Choose the most appropriate expression plasmids with a suitable resistance marker and adjust the composition of the cultivation medium to get a better solubility of recombinant proteins.
By fusing a tagged protein such as glutathione-S-transferase (GST) to the interested protein to achieve higher expression levels and solubility.

Creative Biolabs provides high-quality and efficient service in virus-like particles (VLPs) based on the E. coli cell system. We are very pleased to share our state-of-the-art platforms and professional knowledge with our clients. We have also developed other efficient prokaryotic hosts such as Pseudomonas fluorescens and Lactobacillus casei. Please feel free to contact us for further discussions. We also provide other expression systems including:

VLP Production in Yeast Cell System
VLP Production in Insect Cell System
VLP Production in Plant System
VLP Production in Mammalian Cell System

Reference

Nooraei, Saghi, et al. "Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers." Journal of Nanobiotechnology 19 (2021): 1-27. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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