Stem Cell-derived Exosome Application
- Adipose MSC Source

Introduction Properties Applications Services FAQs

Adipose-Derived Mesenchymal Stem Cell-Derived Exosomes (ADMSC-Exos)

Adipose-derived mesenchymal stem cell-derived exosomes (ADMSC-Exos) represent a promising frontier in regenerative medicine and targeted therapy. As nanoscale extracellular vesicles secreted by adipose-derived mesenchymal stem cells (ADMSCs), these exosomes encapsulate a diverse array of biomolecules, including proteins, lipids, mRNAs, and non-coding RNAs, that mirror the functional signatures of their parent cells. ADMSC-Exos have been shown to participate in fundamental cellular processes through the horizontal transfer of bioactive cargos.

Creative Biolabs focuses on and summarizes the feasibility therapeutic applications of ADMSC-Exos, hoping to help customers open up new research directions. We specialize in exosome isolation and development services, offering customized solutions for both exosome characterization and exosome profiling. Through our comprehensive exosome functional research services in vitro and in vivo, we ensure high-quality ADMSC-Exos for your research and therapeutic needs.

Bidirectional Regulatory Properties of ADMSC-Exos

Masters of Fat Metabolism

ADMSC-Exos are powerful regulators of fat metabolism, but their effects can be surprisingly complex. For instance, they carry miR-125b-5p, which suppresses the browning of white fat and promotes energy storage, a process closely tied to obesity. Conversely, other cargo like transcriptional activator 3 can enhance the browning of white fat, promoting thermogenesis and energy expenditure. Adding to this complexity, miR-199a-3p in ADMSC-Exos has been shown to impair brown adipocyte metabolism. This intricate network of opposing functions highlights the critical need to profile exosomal content carefully before applying them in metabolic research or therapy. Our services like exosomal miRNA sequencing, exosomal RNA isolation and qPCR analysis services and exosome functional research services in vitro and in vivo can be employed for detailed profiling and therapeutic insights.

Fine-Tuning Immune Responses

The immunomodulatory potential of ADMSC-Exos is a major focus of therapeutic interest. These exosomes lack major histocompatibility complex (MHC) class II molecules and co-stimulatory proteins, which limits their capacity to elicit strong immune reactions—an advantage for clinical applications. In addition, they suppress T cell activation by reducing interferon-γ expression and promote anti-inflammatory responses by upregulating interleukin-10 and enhancing macrophage polarization toward an M2 phenotype. This dual regulatory capability suggests that ADMSC-Exos could play a role in dampening excessive inflammation while supporting tissue repair, offering a promising approach for immune-mediated diseases. Our services such as exosomal cytokines profiling service can be leveraged to explore these effects in greater depth.

A Double-Edged Sword in Cancer

The role of ADMSC-Exos in cancer is highly context-dependent, reflecting their complex molecular cargo. In breast cancer, ADMSC-Exos have been shown to promote cell proliferation and migration, potentially contributing to tumor progression. In contrast, in liver cancer models, ADMSC-Exos suppress tumor growth and migration, indicating an inhibitory role. This duality underscores the necessity of detailed profiling—such as miRNA sequencing, exosomal proteomics, and functional assays—before considering ADMSC-Exos as a therapeutic tool in oncology. Understanding the specific cargo and functional properties of ADMSC-Exos is key to unlocking their potential as precision tools for cancer therapy.

Several intrinsic properties distinguish ADMSC-Exos as particularly promising for therapeutic use.

Therapeutic Potential Across Disease Models

The multifaceted therapeutic potential of ADMSC-Exos is reflected across numerous preclinical studies. In the context of cardiovascular disease, ADMSC-Exos have been shown to promote angiogenesis by delivering pro-angiogenic miRNAs that activate endothelial cell function and stimulate vascular remodeling. Similarly, ADMSC-Exos harbor neurogenesis-related cytokines and proteins that support neural differentiation, axonal growth, and synaptic function, offering potential for neuroregenerative therapies.

For tissue repair applications, ADMSC-Exos have demonstrated the ability to suppress osteocyte apoptosis by modulating apoptotic protein expression profiles, as well as to stimulate fibroblast proliferation, migration, and extracellular matrix production via activation of key signaling pathways such as PI3K/Akt. The ability of ADMSCs-Exos in exosome labeling and exosome modification ensures that these cells can target the skin tissue specifically, offering a good therapeutic strategy for both the repair of skin trauma and the improvement of skin texture.

Fig.1 Mechanisms by which ADSCs-EXOs may upgrade wound healing.¹

In liver disease models, engineered ADMSC-Exos loaded with miR-181-5p have been found to activate autophagy-related signaling pathways, alleviating fibrotic progression. In renal injury, ADMSC-Exos attenuate podocyte apoptosis and fibrosis by restraining the Smad1/mTOR axis, offering potential therapeutic value in diabetic nephropathy. Our exosome engineering services , including exosome labeling and exosome cargo loading services, offer precision solutions to optimize the therapeutic properties of ADMSC-Exos for liver damage repair. Furthermore, in myocardial infarction models, ADMSC-Exos enhance M2 macrophage polarization and mitigate cardiac fibrosis via S1P/SK1/S1PR1 signaling, highlighting their cardioprotective potential. Exosome quantification and exosome characterization via nanoparticle tracking analysis and western blotting ensure detailed profiling and analysis of ADMSCs-Exos for effective heart disease treatments.

Notably, ADMSC-Exos have also been investigated as delivery vehicles for therapeutic miRNAs to enhance chemotherapeutic sensitivity in cancer treatment. For instance, ADMSC-Exos loaded with miR-122 have been shown to suppress tumor cell proliferation and metastasis while improving chemotherapy responsiveness. Our disease-targeted exosome modification services, including tumor and cancer-targeted exosome modification, provide the tools needed for efficient delivery and enhanced therapeutic efficacy in cancer treatment.

Tailored Solutions for ADMSC-Exos Research and Development

At Creative Biolabs, we understand the intricate potential of ADMSC-Exos—and the need for precise, reliable research tools to unlock their full therapeutic promise. Our team specializes in a comprehensive suite of exosome-focused services.

Our capabilities include:

State-of-the-art exosome isolation techniques
Advanced exosome characterization (such as nanoparticle tracking analysis and western blotting)
Detailed profiling of exosomal cargos—including miRNAs, proteins, and cytokines
Functional research services both in vitro and in vivo to validate the biological effects of ADMSC-exos in relevant disease models

If you're ready to take the next step in your ADMSC-Exos research, we'd love to collaborate with you. Contact us to learn how our exosome profiling and engineering services can help you push the boundaries of exosome science and unlock new possibilities for disease treatment.

FAQs

Q: Are there risks or limitations when using ADMSC-Exos?

A: While ADMSC-Exos show a lot of promise, they're not a "one-size-fits-all" solution. For example, in some cases, they might promote tissue repair, but in others, they could unintentionally encourage tumor growth or alter immune responses in unwanted ways. That's why thorough characterization—looking at their molecular cargo, surface markers, and functional properties—is crucial before moving into therapeutic applications.

Q: What makes ADMSC-Exos a better option than directly using stem cells?

A: One big advantage is safety. Using whole stem cells can sometimes lead to risks like uncontrolled differentiation or even tumor formation. Exosomes, on the other hand, deliver many of the beneficial molecules from stem cells—like growth factors and regulatory RNAs—without the same safety concerns. Plus, exosomes are more stable, can be stored for longer periods, and are easier to modify for targeted delivery.

Q: Can I use ADMSC-Exos for research in specific diseases, like neurodegeneration or metabolic disorders?

A: ADMSC-Exos have shown promise in a wide range of conditions—everything from wound healing and heart disease to liver fibrosis and neurodegenerative disorders. Their ability to modulate inflammation, promote angiogenesis, and even cross the blood-brain barrier makes them particularly appealing for these types of diseases. If you have a specific disease model in mind, we can help design experiments to test how ADMSC-Exos might work in your system.

Reference

An, Yang, et al. "Exosomes from adipose‐derived stem cells and application to skin wound healing." Cell proliferation 54.3 (2021): e12993. Distributed under Open Access license CC BY 4.0, without modification.