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GTOnco™ Lymphocyte Activation & Proliferation Assay


Quantification of lymphocyte activation and proliferation is a useful way to assess the role played by lymphocytes in an immune response, especially T cells. The lymphocyte activation and proliferation are expected to be earlier indicators of the response to immune stimuli and the related assays have been shown to be useful for monitoring T-cell immune status. At Creative Biolabs, GTOnco™ is able to provide faster, easier-to-use in vivo methods of measuring T-cell activation and proliferation in response to a variety of stimuli. Our services allow studies in an in vivo condition, thus avoiding alterations by functional factors that may appear in vitro, such as the influence of cell types and other factors only found in intact organs. We design assay protocols for customers and select the most appropriate method to advance your gene therapy-based I-O drug development.

Lymphocytes activation is accompanied by a series of intracellular activated events, including the cytokine release and T cell proliferation in order to fight infection or disease. At GTOnco™, we provide cytokine capture assay (IVCCA) by the injection of biotinylated neutralizing anti-cytokine monoclonal IgG antibody to measure the cytokine production in vivo. In addition, by labeling the lymphocytes with fluorescein-based dye, we also offer lymphocyte proliferation in vivo assay. The commonly used agent is carboxy-fluorescein succinimidyl ester (CFSE), which is an effective and popular means to monitor lymphocyte division. Generally, when the labeled cell divides, its progeny are endowed with half the number of carboxy-fluorescein-tagged molecules. At GTOnco™, CFSE-labeled T cells are transferred into animal models and measured through the corresponding decrease in cell fluorescence via flow cytometry. Of course, a broad range of other fluorochromes is supplied to support your projects.

CFSE Labeled-CD8+ T Proliferation Assay. Figure 1. CFSE Labeled-CD8+ T Proliferation Assay. (Quah, 2010)

With professional service and advanced technology platforms at Creative Biolabs, we are committed to providing the most efficient lymphocyte activation & proliferation assay for our clients to best maximize the success of their projects. We are able to conduct projects in standardized procedures that conform to the GLP (FDA/OECD) regulations, which are essential for the IND application. Please don’t hesitate to contact us and let us know your specific requirements.

References

  1. Quah, B. and Parish, C. (2010). The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation. Journal of Visualized Experiments. 44.
  2. Toribio-Fernandez, R.; et al. (2018). An In Vivo Mouse Model to Measure CD4+ T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells. Journal of Visualized Experiments. 138.

For Lab Research Use Only, Not for Human or Animal Therapeutic Use.

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