HCT-8 In Vitro Protein Carbonylation Detection Assay (Oxidative Stress)

This assay is to provide HCT-8-based In Vitro Protein Carbonylation Detection Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HCT-8

Human

HCT-8 [HRT-18] cells were isolated from the large intestine of a 67-year-old, male, adenocarcinoma patient. HCT-8 [HRT-18] are used for cancer and toxicology research.

HRT-18

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Protein Carbonylation Detection Assay (Oxidative Stress)

HCT-8-cell based In Vitro Protein Carbonylation Detection Assay (Oxidative Stress)

Assays for the detection of protein carbonylation have widely been used to determine oxidative stress. Protein carbonylation is one of the irreversible protein modification processes in oxidative stress, which includes modification of side chains of amino acids of proteins to carbonyl derivatives. A wide variety of assays and analytical techniques such as high-performance liquid chromatography (HPLC), gas chromatography, immunoassays, radioactive labeling and capillary electrophoresis have been used to detect protein carbonylation in oxidative stress.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Markers Thiobarbituric Acid Reactive Substances