ModifiSite™ Arginase Inhibitor Screening Kit

CAT#: ITS-1125-WB27
Product Type: Assay Kit
Species: All Species
Target: Manganese-dependent metalloenzyme arginase (ARG1 and ARG2) involved in urea cycle and immune regulation.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies arginase inhibition using a simple colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Arginase Inhibitor Screening Kit provides a sensitive, high-throughput solution for evaluating arginase modulators in drug discovery. This non-radioactive system utilizes a specific chromogenic reaction with urea to determine percent inhibition, supporting the development of therapeutic candidates for cardiovascular and neurological diseases by offering rapid and reliable screening on HTS liquid handling platforms.
Features
Rapid Colorimetric Quantitation: Utilizes an optimized urea-based detection method for high-throughput analysis, delivering reliable absorbance results within 30 minutes.
Exceptional Assay Sensitivity: Features a wide linear range (0.3-20 U/L) ensuring the detection of subtle inhibitory effects in complex libraries.
Interference-Free Performance: Proprietary reagents effectively neutralize background noise from crude extracts, providing superior specificity for target enzyme assessment.
Scalable Homogeneous Format: Designed for seamless integration into automated liquid handling systems, supporting 96-well and 384-well microplate configurations.
Process Relevance
This kit enables precise screening of chemical libraries to identify potent modulators that restore immune response in suppressive environments.
Application Stage
Early-stage drug discovery, high-throughput lead identification, and characterization of candidate selectivity and potency during preclinical optimization.
Applications
Identify novel inhibitors, determine IC50 values, screen natural products, and characterize arginase activity in tumor microenvironments, immune cell supernatants, and purified protein preparations.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Manganese-dependent metalloenzyme arginase (ARG1 and ARG2) involved in urea cycle and immune regulation.
Detection Method
Absorbance at 430 nm
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, immunology, cardiovascular physiology, metabolic disorders, nitrogen metabolism, etc.
Size
100 tests
Data Output Type
Quantitative measurement of enzyme inhibition using endpoint absorbance (OD 520 nm) or kinetic monitoring.
Assay Time
105 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Arginase facilitates the final step of the urea cycle by hydrolyzing L-arginine into urea and ornithine. Its overexpression in tumor-associated macrophages depletes local arginine, effectively paralyzing T-cell-mediated anti-tumor immunity.
Pathways
Arginase operates within the urea cycle and L-arginine metabolism. It competes with nitric oxide synthase (NOS) for substrates, modulating nitric oxide production and polyamine synthesis downstream.
For Research Use Only | Not For Clinical Use
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