Short Description
Quantifies arginase inhibition using a simple colorimetric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Arginase Inhibitor Screening Kit provides a sensitive, high-throughput solution for evaluating arginase modulators in drug discovery. This non-radioactive system utilizes a specific chromogenic reaction with urea to determine percent inhibition, supporting the development of therapeutic candidates for cardiovascular and neurological diseases by offering rapid and reliable screening on HTS liquid handling platforms.
Features
Rapid Colorimetric Quantitation: Utilizes an optimized urea-based detection method for high-throughput analysis, delivering reliable absorbance results within 30 minutes.
Exceptional Assay Sensitivity: Features a wide linear range (0.3-20 U/L) ensuring the detection of subtle inhibitory effects in complex libraries.
Interference-Free Performance: Proprietary reagents effectively neutralize background noise from crude extracts, providing superior specificity for target enzyme assessment.
Scalable Homogeneous Format: Designed for seamless integration into automated liquid handling systems, supporting 96-well and 384-well microplate configurations.
Process Relevance
This kit enables precise screening of chemical libraries to identify potent modulators that restore immune response in suppressive environments.
Application Stage
Early-stage drug discovery, high-throughput lead identification, and characterization of candidate selectivity and potency during preclinical optimization.
Applications
Identify novel inhibitors, determine IC50 values, screen natural products, and characterize arginase activity in tumor microenvironments, immune cell supernatants, and purified protein preparations.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Manganese-dependent metalloenzyme arginase (ARG1 and ARG2) involved in urea cycle and immune regulation.
Detection Method
Absorbance at 430 nm
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, immunology, cardiovascular physiology, metabolic disorders, nitrogen metabolism, etc.
Datasheet