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TGFB2

Products

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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Background

Transforming growth factor beta 2 (TGFB2) is a 25 kDa secreted cytokine member of the TGF-β superfamily of growth and differentiation factors. Furin-like proprotein convertases cleave the precursor into two different chains, which are the N-terminal latency-associated peptide (LAP) and C-terminal mature bioactive peptide. Post-cleavage, these chains stay non-covalently associated, providing a latent, inactive cytokine during matrix storage and transport. Mature TGFB2 peptide is a disulfide-linked homodimer that has pleiotropic effects on most if not all cell types in the body. Latent TGFB2 can be activated through context-dependent extracellular mechanisms that release or expose the mature cytokine from its latent complex. Once activated, dimeric TGF-β binds a receptor complex formed by type II and type I receptors. The interaction results in trans-autophosphorylation of TGFBR2, which then phosphorylates TGFBR1, activating the serine/threonine kinase domain. The downstream signalling cascade involves SMAD2/3 phosphorylation, nuclear translocation and transcriptional regulation of target genes associated with cell proliferation, apoptosis, extracellular matrix deposition, and immune suppression. In addition, the mature peptide has been shown to heterodimerize with TGFB1 and TGFB3, further increasing the combinatorial milieu in which they can signal.

Fig.1 Opposite effects of increasing TGFB2 and IFNGR2/JAK1/STAT1 mRNA levels. (OA Literature)Fig.1 Opposite effects of increasing TGFB2 and IFNGR2/JAK1/STAT1 mRNA levels on OS outcomes in pbDMG patients.1

TGFB2 Protein Function: A Master Orchestrator of Tissue Homeostasis and Pathological Remodeling

TGFB2 plays a biological role in development, immunity and disease:

  • Immune Regulation and Tolerance: TGFB2 is a potent inhibitor of interleukin-2 dependent T-cell proliferation and has been implicated as an important mediator in the differentiation of regulatory T-cells. It induces FOXP3 expression and suppresses effector T-cell responses, thus promoting peripheral immune tolerance.
  • Fibrosis and Extracellular Matrix Deposition: TGFB2 is a strong pro-fibrotic stimulus in organ systems. It is a potent stimulator of fibroblast contractile activation to myofibroblasts, regulates collagen and fibronectin synthesis negatively mediated by matrix metalloproteinases activity leading to pathological scarring at lung, liver, kidney outside eye & skin.
  • Angiogenesis and Vascular Development: TGFB2 is involved in regulating heart development in the embryo and blood vessel morphology. In adults it adjusts migration of endothelial cells, pericyte recruitment and the stability of vessels.

TGFB2 Protein Product

Boost your research with our curated portfolio of recombinant TGFB2 proteins. As a secreted cytokine, TGFB2 requires appropriate folding, disulfide bond formation, and structural integrity to support studies of its latent and active states. We provide TGFB2 protein formats designed for a range of research applications, including receptor-binding studies, antibody screening and characterization, signaling research, and drug discovery. Specific protein formats, expression systems, quality-control specifications, and available bioactivity data are provided according to the corresponding product datasheet or individual project requirements.

TGFB2 Protein Product

Not finding the protein product you need? Contact us to start your one-stop custom service!

TGFB2 Stable Cell Line Product

Developing robust, physiologically relevant cellular platforms that can recapitulate TGFB2-driven biology and assess anti-fibrotic candidates are key. Here we describe the engineering of stable cell lines that exclusively express human, mouse or rat TGFB2 as a single isoform or in physiologically relevant latent complexes in fibroblast, epithelial, or immune cell backgrounds either constitutively or inducibly. They have been optimized and standardized for differentiation assays relevant to myofibroblast (MF) biology, quantification of collagen deposition upon TGFbeta2 stimulation, SMAD reporter gene assay analysis as well as high-throughput screening of both TGFB2-neutralizing antibodies and receptor kinase inhibitors with the reproducibility needed for multi-phase studies performed by multiple research groups.

TGFB2 Stable Cell Line Product

Not finding the stable cell line product you need? Contact us to start your one-stop custom service!

TGFB2 Recombinant Antibody Product

One of the most thoroughly tested recombinant antibody collections specifically for TGFB2, to ensure accurate TGFB2 detection and strong quantification. Developed from prominent techniques in the immunology field, they are incredibly specific for unique TGFB2 epitopes (the mature peptide and the latency-associated peptide) and having lower cross-reactivity with TGFB1 or TGFB3. Well validated for numerous applications Types Test validation: WB, ELISA, FCM, IF, ICC, IHC and IP — read more Well characterized (strong specificity) against TGFB2 across fibrotic tissues, tumor microenvironments and serum samples.

TGFB2 Recombinant Antibody Product

Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!

Product Features

  • High Purity & Activity: High purity and verified integrity for dependable downstream work.
  • Diverse Expression Systems: Choice of expression formats to match routine or specialized projects.
  • Species Reactivity: Products available for human, mouse, pig, and other species.
  • Validated Applications: Application-tested to deliver consistent signals across common lab workflows.
  • Superior Batch-to-Batch Consistency: Stringent quality controls to ensure low variability from batch to batch.
  • Customization Options: Tags/Labels/package Modifications available upon request.

Custom TGFB2 Membrane Protein and Antibody Discovery Services

We also provide full-service custom services in addition to our catalog products, focused on expediting TGFB2-targeted drug discovery and biomarker development:

  • Production & Validation: Cloning of the synthesized optimized gene into an expression vector, plus small-scale expression trials to help determine the best expression conditions.
  • Custom Antibody Production: Produced via large-scale expression in mammalian/insect/cell culture or bacterial expression systems, purified in detergent or reconstituted into nanodiscs.
  • Engineered Cell Lines: Creation of stable cell lines that physiologically mimic endogenous TGFB2 expression and activity.
  • Functional Assessment Systems: Designing and executing assays to assess protein function and ligand binding.

Frequently Asked Questions (FAQ)

  1. Are your TGFB2 products approved for clinical or diagnostic use?

    No. All TGFB2 recombinant proteins, cell lines and antibodies are for research use only.

  2. Do you have systems for stable cell lines with inducible TGFB2 expression or TGFBR1/ALK5 co-expression?

    Absolutely. We prior have cell engineering team constantly developed tetracycline-inducible systems and lines co-expressing TGFB2 with TGFBR2, TGFBR1 or SMAD2/3 reporter constructs for signalational analysis.

  3. Do you have stable cell lines co-expressing mature TGFB2 along with TGFBR1 and TGFBR2 that allow long-term modeling of autocrine Smad signaling &epithelial-to-mesenchymal transition?

    Yes. We generated triple-stable lines with constitutive expression of mature TGFB2, TGFBR1 and TGFBR2 from independent loci. As the data for each batch is released showing Smad2 phosphorylation and fibronectin induction, these provide a repeatable cellular platform that allows continuous mechanistic studies without re-performing transient transfection.

  4. Do you also generate TGFB2 variants that carry point substitutions in the receptor-binding interface that completely abolish TGFBR2 engagement for dominant-negative specificity control?

    Yes. We provide a unique set of recombinant proteins as well as stable cell lines that express variants containing alanine substitutions at key positions in the hydrophobic receptor binding pocket domain required for type II receptor protein-protein interaction.

Reference
  1. Qazi, Sanjive, Zahra Talebi, and Vuong Trieu. "Transforming growth factor beta 2 (TGFB2) and interferon gamma receptor 2 (IFNGR2) mRNA levels in the brainstem tumor microenvironment (TME) significantly impact overall survival in pediatric DMG patients." Biomedicines 12.1 (2024): 191. Under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/biomedicines12010191
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