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TNFRSF1A

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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Background

TNFRSF1A is also known as TNFR, p55 or CD120a685, which is expressed in nearly all tissues of human origin, notably within immune cells or their precursors as well as endothelial cell s(ECs), fibroblast s and neutrons of several organs including liver. The wide distribution in the body serves as a rationale for pleiotropic functions of TNF-α/TNFR1 signaling at various stages including rapid host defense, tissue repair and immune surveillance. How to tag it Germline mutations in the extracellular domain of TNFRSF1A cause a well-established (but less common) disorder: tumor necrosis factor receptor--associated periodic syndrome TRAPS is an autosomal dominant autoinflammatory disease that presents with recurrent fevers, abdominal pain, migratory rash and periorbital edema (potentially leading to ocular damage), markedly variable expression due pon patchy penetrance; increased risk for secondary amyloidosis. To date, over 70 variants associated with TRAPS are identified, which were responsible for inhibited receptor shedding or aberrant intracellular trafficking then elevated surface expression to enhance TNF-α hyperresponsiveness. Besides monogenic disease, dysregulated TNFR1 signalling drives rheumatoid arthritis, psoriasis/psoriatic arthropathy ankylosing spondilitis ( AS), familial Crohn's disease (IBD) and ulcerative colitis IBD combined sporadic multiple sclerosis non-small cell lung cancer pancreatic ductal adenocarcinoma.

Fig.1 Network of the TNF-α signaling pathway via TNFRSF1A. (OA Literature)Fig.1 Reconstruction of the small gene network of the TNF-α signaling pathway from TNFR1 and TNFR2 in K562.1

TNFRSF1A Receptor Function: A Bifurcated Switch Between Inflammation and Cell Death

The functional scope of TNFRSF1A crosses many physiological and pathological domains, echoing its ability to promote survival or destruction:

  • NF-κB–Driven Inflammation and Immunity: TNFR1 activates the canonical NF-κB pathway through complex I assembly, inducing transcription of pro-inflammatory cytokines (IL-6, IL-lβ, TNF-α itself), chemokines and adhesion molecules. This response is critical for antimicrobial defense and adaptive immune system priming.
  • Apoptotic and Necroptotic Cell Death: Following the inhibition of complex I signaling, TNFR1 internalization allows for the assembly of a secondary group called complex II which results in FADD-mediated caspase-8 activation. If caspase-8 is inactive, RIPK1 recruits RIPK3 to mediate MLKL-dependent necroptosis: a lytic form of regulated cell death underlying tissue damage in inflammatory diseases.
  • Receptor Shedding and Inflammatory Regulation: TACE/ADAM17 mediated ectodomain shedding generates sTNFR1 which neutralise serum TNF-α, forming a negative feedback loop—impaired receptor sheddding (common to TRAPS mutations) triggers prolonged receptor signalling enhancing inflammatory periodes.

TNFRSF1A Membrane Protein Product

The complexities of TNFRSF1A, including ligand-dependent trimerization, PLAD-mediated pre-assembly, death domain–adaptor interactions, and sensitivity to metalloprotease shedding, complicate structural and pharmacological characterization. To address these challenges, Creative Biolabs has developed a custom TNFRSF1A protein design platform that provides receptor constructs suitable for structural biology, ligand-binding studies, and signaling research. Using structural insights and codon-optimized synthetic genes, our engineering team develops constructs tailored to specific experimental requirements while considering key structural and functional regions of TNFRSF1A. Every engagement begins with a thorough technical consultation to ensure that the protein architecture fits the intended application. Specific receptor formats, interaction interfaces, preparation strategies, and functional assays are determined according to individual project requirements.

TNFRSF1A Protein Product

Not finding the membrane protein product you need? Contact us to start your one-stop custom service!

TNFRSF1A Stable Cell Line Product

Functional cellular models expressing TNFRSF1A on the cell surface can support agonist/antagonist screening, antibody evaluation, and studies of receptor-mediated signaling. Using optimized transduction and selection protocols, Creative Biolabs engineers custom TNFRSF1A stable cell lines with sustained receptor expression. The cell engineering platform utilizes lentiviral delivery, transposon-mediated integration, and targeted knock-in approaches depending on project requirements. Generated TNFRSF1A cell models may support studies of TNF responses, downstream signaling, and receptor shedding. Specific expression characteristics, phenotypic validation, and functional performance are documented according to the corresponding cell-line datasheet or individual project requirements.

TNFRSF1A Stable Cell Line Product

Not finding the stable cell line product you need? Contact us to start your one-stop custom service!

TNFRSF1A Recombinant Antibody Product

To detect TNFRSF1A in inflamed tissue, delineate its surface distribution across cell types, selectively antagonize TNFR1 but not TNFR2 and neutralize the therapeutic effects of excess solubleTNF-αin vivo requires high-affinity sequence-defined antibodies to prevent receptor dimerization. We provides end-to-end recombinant antibody discovery programs focused on PLAD and CRD - Helix 2 regions of TNFRSF1A epitopes (extracellular & conformation directed). The pipeline includes immunogen design, multi-platform selection and downstream engineering to produce binders with the desired specificity affinity and developability profiles for research diagnostic or therapeutic applications. Using recombinant expression and clonal sequencing, we remove the batch-to-batch variability seen with conventional polyclonal sera to provide you a renewable fully characterized reagent.

TNFRSF1A Recombinant Antibody Product

Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!

Product Features

  • High Purity & Activity: High purity and verified integrity for dependable downstream work.
  • Diverse Expression Systems: Choice of expression formats to match routine or specialized projects.
  • Species Reactivity: Products available for human, mouse, pig, and other species.
  • Validated Applications: Application-tested to deliver consistent signals across common lab workflows.
  • Superior Batch-to-Batch Consistency: Stringent quality controls to ensure low variability from batch to batch.
  • Customization Options: Tags/Labels/package Modifications available upon request.

Custom TNFRSF1A Membrane Protein and Antibody Discovery Services

Partnering with Creative Biolabs for TNFRSF1A protein engineering offers differential access to a vertically integrated platform underpinned by deep death receptor immunology expertise and best-in-class quality standards:

  • Production & Validation: Cloning of the synthesized optimized gene into an expression vector, plus small-scale expression trials to help determine the best expression conditions.
  • Custom Antibody Production: Produced via large-scale expression in mammalian/insect/cell culture or bacterial expression systems, purified in detergent or reconstituted into nanodiscs.
  • Engineered Cell Lines: Creation of stable cell lines that physiologically mimic endogenous TNFRSF1A expression and activity.
  • Functional Assessment Systems: Designing and executing assays to assess protein function and ligand binding.

Frequently Asked Questions (FAQ)

  1. Are TNFRSF1A variants associated with TRAPS available for comparative signaling studies?

    Yes, we provide recombinant proteins and stable cell lines with known mutations in the extracellular cysteine-rich domains that can be tested directly for comparison of ligand-binding affinity, receptor oligomerization or inflammatory signaling output to those from wildtype controls.

  2. Do you provide your recombinat TNFRSF1A ectodomain in the formulation directly suitable for surface plasmon resonance or biolayer interferometry?

    Yes, the protein is buffered at defined conditions without carrier proteins or primary amines in an appropriate buffer with immediate immobilization to sensor chips for TNF-α binding kinetics and therefore can be used directly after its purification without dialysis.

  3. Are your TNFRSF1A antibodies cross-reactive with mouse and rat orthologs?

    Yes, the Immunogen binds to a conserved region. This provides consistent detection across human, mouse and rat preparations without species-specific reagents.

  4. Do you offer stable cell lines that co-express TNFRSF1A and TRADD for analyzing death-inducing signaling complex assembly?

    Certainly, we have established two dual-stable lines constitutively expressing both full-length TNFRSF1A and TRADD where DISC formation & NF-κB activation kinetics can be assessed directly avoiding the variability associated with transient transfections.

Reference
  1. Perik-Zavodskaia, Olga, et al. "Knockouts of TNFRSF1A and TNFRSF1B genes in K562 cell line lead to diverse long-lasting responses to TNF-α." International Journal of Molecular Sciences 24.24 (2023): 17169. Under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/ijms242417169
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