Immune Synapse Formation Assay

Why do We Study Immune Synapse Formation?

The immunological synapse (IS) is a specialized intricate supramolecular activation cluster structure that arises at the interface between T cells and antigen-presenting cells. Its formation is an essential step in humoral immune responses, which supports the integrity of the contact between T cells and APC, and stimulates T-cell activation and proliferation. The establishment of an immune synapse formation assay platform is of the utmost importance for revealing the pathogenesis of disease and new drug development.

Fig.1 Immune synapse formation and T-cell activation. (Quintana, 2007)

How to Study Immune Synapse Formation?

Creative Biolabs provides high-quality immune synapse formation assay services to investigate IS formation and how various chemicals and therapies may influence it.

In our immune synapse formation assay, we provide a deeper look at the interaction between T cells and target cells to analyze the impact of test articles and the immunotherapies on the formation of the immune synapse through a cell culture model. T cells were pre-stimulated and then fluorescently labeled, while APC or target cells were pre-cultured, and then co-culture two cell types and add relevant test articles. Some special markers like LFA-1, ICAM-1, and Phalloidin were used to identify synapses, and DAPI nuclear staining was used to accurately count the total number of cells. The final detection parameters can be given by the imaging instrument. Throughout the process, factors such as treatment concentration, and incubation time, might influence the final results. Creative Biolabs optimizes the entire experiment based on the customer's project.

Fig.2 Imaging secretory traffic polarization at immune synapses of living T lymphocytes. (Calvo, 2018)

Service Workflow

Creative Biolabs is dedicated to providing high-quality immune synapse formation assay services and delivering accurate reports to our customers.

Fig.3 Our immune synapse formation assay workflow. (Creative Biolabs)

Highlights of Our Services

Multiple cell types to empower our immune synapse formation assay service.

We have 200+ human tumor cell lines to choose from for immune synapse formation assay.

Extensive immune-oncology experience to empower immune synapse formation assay service.

We have experienced immunologists to help carry out relevant experimental projects in the immune-oncology field.

Standardized and strict quality control to achieve high reliability of data

We follow strict standards from experimental design, and experimental procedures, to final data processing to achieve reliable service quality.

Representative Data

1. The immune synapses reveal the CD8 T cells' aberrant functions during chronic HIV infection

Fig.4 Diverse synaptic interfaces established from Human polyclonal CD8 T cells with lipid bilayers. (Anikeeva, 2022)

2. LPA inhibits T cell function by changing the cytoskeleton and disrupting immune synapse formation

Fig.5 IP3R1 localizes to the immune synapse of antigen-specific conjugates. (Kremer, 2022)

For more details about our immune synapse formation assay service, please don't hesitate to contact us for more information.

References

1. Quintana A., et al. T cell activation requires mitochondrial translocation to the immunological synapse. Proc Natl Acad Sci U S A. 2007, 104(36): 14418-23.
2. Calvo V.; Izquierdo M. Imaging polarized secretory traffic at the immune synapse in living T lymphocytes. Front Immunol. 2018, 9: 684.
3. Kremer K.N., et al. LPA suppresses T cell function by altering the cytoskeleton and disrupting immune synapse formation. Proc Natl Acad Sci U S A. 2022, 119(15): e2118816119.
4. Anikeeva, N., et al. The immune synapses reveal aberrant functions of CD8 T cells during chronic HIV infection. Nat Commun. 2022, 13(1): 6436.

For Research Use Only.