After years of experience in antibody discovery and manufacture, Creative Biolabs has established a distinguished antibody functional assay platform. We are proud to offer a comprehensive package of immune checkpoint functional assays in a timely and cost-effective manner. Here, we are happy to introduce our high-quality T cell cytotoxicity assay for our clients all over the world.
Cytotoxic T lymphocytes (CTL) are important effector cells of the immune system. By killing target cells on the basis of cell-surface antigen recognition, they play important roles in the host immune response to tumors, transplants, and viruses. Studies have shown that T cells perform their functions by elaborating a number of distinct effector mechanisms. What’s more, in vitro cytotoxicity assays have shown the presence of two major contact-dependent cytotoxic pathways. First, cytotoxic effect cells can secrete lytic granules, comprising a pore-forming toxin, perforin, and pro-apoptotic serine proteases, granzymes, which synergistically kill target cells by activating various lytic pathways. Second, effector cells produce the TNF family members, such as TNF-α, Fas ligand (FasL) or TRAIL, thereby inducing multimerization of their cognate receptors on target cells and leading to apoptosis.
Fig. 1 Cytotoxic functions of CD8 T cells.1
Traditionally, scientists use MTT, CCK8, and CFSE-PI to detect cytotoxicity. However, as T cells also undergo apoptosis when incubated with tumor cells, these methods are unable to detect T cell cytotoxicity. With years of experience and advanced technologies, Creative Biolabs has successfully established an advanced platform which can analyze the potential of antibody candidates to modulate immune effector cells by killing tumor cells in a real-time, label-free manner. Our services include long-term cytotoxicity assays to reveal T cell killing in cell cultures and drug-mediated cytotoxic effects. Particularly, our experiments can run for several days, which makes this cytotoxicity assay much more sensitive than traditional methods such as the chromium release assay. Furthermore, we can make use of a broad range of cell lines derived from both cancer and healthy patients.
To meet every client’s specific requirements, Creative Biolabs provides both standard and customized strategies for your immune checkpoint functional analysis. If you are interested in the service we provide, please feel free to contact us for more detailed information.
Reference
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