In Vitro Non-Antigen Specific Functional Assays

With unrivaled expertise and experiences in immunology discovery and development, Creative Biolabs is confident in providing in vitro non-antigen specific functional assay services to best suit your program requirements.

Background of Non-Antigen Specific Functional Assays

Non-antigen specific functional assays have been used for in vitro pharmacodynamic evaluation, such as assessing the potential of T cell responses in biologic. With the development of therapeutics and drugs, non-antigen-specific functional assays are increasingly being recognized as a critical part of non-clinical characterization of therapies.

PHA or SEB Stimulation Function Assay

Generally, non-antigen specific functional assays include Staphylococcal enterotoxin B (SEB) or Phytohaemagglutinin (PHA) stimulation functional assays. These stimulation functional assays have been widely used in immunology projects, with well-characterized potent mode of action and relatively inexpensive cost compared to using monoclonal antibodies. SEB and PHA are known as superantigens, which can trigger polyclonal T cell activation and multiple cytokine release. When the events occur, resulting the up-regulation of activation markers and proliferation of T cells, which can be detected by flow cytometry. In addition, the stimulation assay leads to multiple systemic releases of pro-inflammatory cytokines, such as TNF-α, IFN-γ, IL-2, and IL-6, which can be measured by cytokine release assays using cytometric bead arrays or ELISA method.

Fig.1 CD69 expression following staphylococcal enterotoxin B (SEB) stimulation. (Rudolph, 2018)

Our In Vitro Non-Antigen Specific Functional Assays

Leveraging our professional knowledge and experience, Creative Biolabs is confident in performing in vitro non-antigen specific functional assays to meet global clients' needs. We have established and optimized critical factors and provide high-quality in vitro non-antigen specific functional assays. Our experienced immunologists are committed to following up on each step of the project's experimental design to ensure reliable and repeatable data.

In our assays, we fully ensured that the PBMCs or whole blood used in the project were derived from healthy donors. At the same time, we will use reagents and consumables with strict quality control. More importantly, we will analyze and optimize the experimental scheme to ensure the clients' needs can be met properly.

Creative Biolabs is confident in providing comprehensive in vitro functional assay services of high quality to meet clients' demands. We can provide the following assays for global clients, including but not limited to:

Cytokine Release Assay

T Cell Cytotoxicity Assay

Mixed Lymphocyte Reaction (MLR) Assay

For more details on our In vitro functional assay portfolio services, please don't hesitate to contact us for more information.

Reference

1. Rudolph, M. E., McArthur, M. A., et al. Differences between pediatric and adult T cell responses to in vitro staphylococcal enterotoxin B stimulation. Frontiers in immunology. 2018 Mar 20; 9:498.

For Research Use Only.