T Cell Proliferation Assay
Creative Biolabs provides comprehensive antibody function analysis services to make the best understanding of interested antibodies, including both the Fab fragment and the Fc fragment. Particularly, we offer tailored solutions for T cell proliferation assay to help global customers discover potential immune checkpoint function.
Background
T cells play a critical role in triggering adaptive immune responses by recognition of antigens. Importantly, the adaptive immune response improves with time and results in the generation of immunological memory and long-lasting protection. During the past years, a series of immunotherapy approaches aiming at inducing and engineering T cell responses have been developed for the treatment of multiple diseases, such as cancer and autoimmune diseases. Once the immune response is triggered, activated immune cells, such as T cells, undergo rapid expansion to fight against infections or diseases. Interactions between activated immune cells also occur and become the fundamental of regulating the effect and extent of the immune response. To help global clients understand the mechanisms of T cell activation and proliferation, Creative Biolabs offers high-quality T cell proliferation assays to promote the development of novel and effective anti-tumor immunotherapeutics.
Fig.1 Schematic representation of CD137+ T-cell activation as a result of priming by TAA-carrying activated APC.1, 2
T Cell Proliferation Assay at Creative Biolabs
As one of the most common ways to measure T cell activation in vitro, T cell proliferation assay has become an important tool for investigating the functional competence of the specific immune system in patients suffering from HIV, cancer, and recurrent viral or intracellular bacterial infections. In this field, Creative Biolabs is capable of offering cost-effective T cell-based 96-well plate assays to evaluate the effects of immunomodulatory compounds/antibodies alone, or in combination with specific antibodies, on immune cell activation. By real-time imaging T-cells' phenotype changes in response to antibody candidates, we can directly analyze their ability to restore T-cell functions. Meanwhile, detailed kinetic data and time-dependent effects can also be obtained, which are of great significance for future potency characterization.
Advanced Technologies for Effective T cell Proliferation Assay
Our advanced platform integrates cutting-edge technologies to assess T-cell proliferation and evaluate responses to both agonist and antagonist stimuli. Below are some optimized techniques frequently utilized by Creative Biolabs for T-cell proliferation assays.
- Cell counting kit-8 (CCK-8)
- MTT assay
- BrdU incorporation assay
- 3H-TdR incorporation assay
- Live cell CFSE staining flow cytometric
Features of Our Services
- T cells from various origins (PBMCs, purified populations, etc.)
- Real-time phenotype & kinetic monitoring by live cell time-lapse imaging
- Both standard and customized assays for peptide epitope mapping and whole protein screening
- Comprehensive study report with reproducible data
Frequently Asked Question
- Q1: What are the requirements for compound preparation?
Based on our extensive experience and advanced platforms, Creative Biolabs is proud to provide T cell proliferation assays for our customers all over the world. Our service can be tailor-designed to meet your specific needs. If you are interested in our services, please do not hesitate to contact us for more details.
References
- Ugolini, Alessio, and Marianna Nuti. "CD137+ T-cells: Protagonists of the immunotherapy revolution." Cancers 13.3 (2021): 456.
- Distributed under Open Access License CC BY 4.0, without modification.
For Research Use Only.