Macrophage/Monocyte Inflammation Stimulation Assay Service

Background of Macrophage / Monocyte

Monocytes and macrophages play crucial roles in the innate immune system, contributing to various physiological functions such as tissue maintenance, pathogen defense, and immune response regulation. Upon encountering inflammatory stimuli, these versatile cells display remarkable plasticity, adapting and transforming into distinct subsets based on the surrounding microenvironment. Creative Biolabs provides a macrophage/monocyte inflammation stimulation assay service to advance therapeutics development.

Fig. 1 Macrophages/monocytes: key players of the innate immune system.¹

Our Macrophage/Monocyte Inflammation Stimulation Assay Service

Our macrophage/monocyte inflammation stimulation assay service leverages advanced expertise and technologies to evaluate the effect of candidate compounds on macrophage/monocyte inflammation in a gradient-dependent manner. We provide various ready-to-use PBMCs and also are able to isolate PBMCs from fresh blood. Of course, if customers have unique requirements, we have the capacity to customize your experiment scheme.

For the inflammation examination, we offer cutting-edge technologies based on our well-equipped platform to analyze cytokine release, cell chemotaxis, etc. Throughout the process, Creative Biolabs provides a full range of services encompassing cell preparation, inflammation induction, compound incubation, inflammation analysis, and results visualization.

Fig. 2 Workflow of our macrophage/monocyte inflammation stimulation assay.

Intracellular Flow Cytometry Antibodies for Our Macrophage / Monocyte Inflammation Stimulation Assay Service

Alternative Fluorescent Dye for Flow Cytometry

In addition to antibody staining, the fluorescent dye is an alternative option for macrophage/monocyte inflammation stimulation evaluation. Creative Biolabs brings out a number of fluorescent dyes to design for customers' projects with diverse demands:

• Organic small molecules: Fluorescein.
• Phycobilin.
• High molecular dye.
• Fluorescent protein: GFP; mBanana.
• Nucleic acid dye: Propidium iodide.
• Reactive dye: Propidium iodide, DAPI.
• Calcium indicator dye: indo-1; Fluo-3.

Benefits for You

• High throughput.
• Highly reproducible.
• Rapid timelines.
• Customized service.
• Strict quality control.

Frequently Asked Questions

Q1: How to stimulate monocytes and macrophages in this service?

A1: Our service takes advantage of LPS, bacteria, pro-inflammatory cytokines, and other factors to trigger the inflammation reaction of macrophages/monocytes. At the same time, we treat macrophages/monocytes with customers' candidate samples. By monitoring the inflammation variation, we evaluate the effectiveness and efficacy of your candidate samples in the laboratory setting.

Q2: What are the requirements for samples in macrophage/monocyte inflammation stimulation assay service?

A2: We need customers to provide some basic information, such as testing samples, molecular weight, concentration, etc. For PBMC, customers are capable of executing your projects with PBMCs provided by Creative Biolabs, or you supply us with selected PBMCs. Noteworthy, when you transport your selected PBMCs, it is possible to cause cell death or viability decreased. Furthermore, if your projects are characterized by special requirements, please contact our team of experts directly to help you.

For more details about our macrophage/monocyte inflammation stimulation assay service, please feel free to get in touch with us.

Reference

1. Austermann, Judith, Johannes Roth, and Katarzyna Barczyk-Kahlert. "The good and the bad: Monocytes’ and macrophages’ diverse functions in inflammation." Cells 11.12 (2022): 1979.
   Distributed under Open Access License CC BY 4.0, without modification.

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