In Vitro Treg Suppression Assays

Based on profound expertise and professional service, Creative Biolabs is confident in providing in vitro Treg suppression assays services to facilitate your project's success.

Background

In the immune system, regulatory T cells (Tregs) play a crucial role in the prevention of several diseases, such as autoimmune diseases, allergies, infection-induced organ pathology, transplant rejection as well as graft versus host disease (GvHD), by suppressing effector T cells and other immune cells. The suppression of inappropriate immune responses by Treg cells is one of the main ways to maintain immune tolerance and homeostasis. Since the deficiencies in the suppressive capacity of Treg cells is the basis of many different immune-mediated diseases, it is of great significance to develop methods to study the mechanism of Treg function.

Fig. 1 Treg suppressive mechanisms. (Romano, 2019)

Our In Vitro Treg Suppression Assays

In order to better study the potential therapeutic reagents and related mechanism of T cells (Tregs) related action, Creative Biolabs has developed and optimized a series of functional assay platforms to provide relevant experiments. With our extensive knowledge, Creative Biolabs has developed and optimized in vitro Treg suppression assays with quality-controlled Tregs, stimulation reagents, and responder cells. In addition, we can also customize suitable experiments for clients to accelerate the research process.

In our assays, the Tregs and responder cells are isolated and purified from healthy human PBMC, and staining with antibodies for flow cytometric analysis. Tregs and responders seeded at different ratios (depending on the specific project) in the presence of stimulation reagents and the Treg-targeted molecules. After the co-culture, the cells are harvested and analyzed for the proliferation function.

Our Strengths

• Compelling offering on expertise and customized assay solutions.
• An excellent expert team for providing real-time technical guidance.
• Guaranteed service: our assays are performed with high-quality cells and strict controls by professional immunologists.

Creative Biolabs offers comprehensive immunoassay portfolio services for global customers. We can provide the following assays for our clients, including but not limited to:

T Cell Proliferation Assay

Cytokine Release Assay

T Cell Cytotoxicity Assay

For more detailed information, please feel free to contact us or directly sent us an inquiry.

Reference

1. Romano M, Fanelli G, et al. Past, present, and future of regulatory T cell therapy in transplantation and autoimmunity. Frontiers in immunology. 2019 Jan 31; 10:43.

For Research Use Only.