What types of cells can be used in Creative Biolabs' In Vitro Treg Suppression Assay?

We can utilize a variety of cell sources, including fresh or cryopreserved human and mouse Peripheral Blood Mononuclear Cells (PBMCs), isolated CD4+ T cells, and specific Treg subsets. We also accommodate client-provided cells.

How is the suppressive capacity of Tregs measured in the assay?

Treg suppressive capacity is typically measured by assessing the inhibition of the proliferation of responder T cells. We use advanced flow cytometry with proliferation dyes to precisely quantify cell division, providing clear, quantifiable suppression percentages.

Can Creative Biolabs customize the assay to fit my specific research needs?

Absolutely. Our In Vitro Treg Suppression Assay is highly customizable. We can adjust cell ratios, stimulation methods, incubation times, and readouts to align perfectly with your unique experimental design and research objectives.

In Vitro Treg Suppression Assay Service

Based on profound expertise and professional service, Creative Biolabs is confident in providing in vitro Treg suppression assay services to facilitate your project's success.

The Indispensable Role of Tregs in Immune Homeostasis

Regulatory T cells (Tregs) are essential regulators of the immune system, actively preventing diseases like autoimmune disorders, allergies, infection-induced pathologies, transplant rejection, and graft-versus-host disease (GvHD) by suppressing effector immune cells. Their ability to maintain immune tolerance and homeostasis through targeted suppression of inappropriate responses is critical. Given that deficiencies in Treg suppressive capacity underlie numerous immune-mediated diseases, developing robust methods to understand and analyze Treg function is of paramount significance for therapeutic advancement.

Fig.1 Treg suppressive mechanisms. (OA Literature) Fig.1 The mechanisms of Treg suppression.¹

In Vitro Treg Suppression Assay at Creative Biolabs

Creative Biolabs is dedicated to providing tailored solutions for your projects by precisely evaluating the suppressive capacity of regulatory T cells (Tregs). Our optimized in vitro Treg suppression assays leverage quality-controlled Tregs, specific stimulation reagents, and highly responsive cells to provide accurate and reliable insights. We offer customized experimental designs to align with your unique research goals, ensuring the swift progression of your studies.

Our assays involve the isolation and purification of Tregs and responder cells from healthy human PBMCs, followed by flow cytometric analysis. These cells are then co-cultured at project-specific ratios with appropriate stimulation and Treg-targeted molecules. Post-co-culture, we analyze cell proliferation to quantify the precise suppressive function of Tregs. Creative Biolabs guarantees the quality of results through stringent quality control measures at each step, using high-purity cells, optimized reagents, and state-of-the-art analytical platforms, ensuring robust and reproducible data for your critical research.

Required Starting Materials

To initiate the service, clients typically provide:

Isolated PBMCs or specific T cell subsets: From human or animal samples, ideally cryopreserved or fresh.
Test compounds or biological agents: To investigate their impact on Treg function.
Project specifications: Including desired readouts, co-culture ratios, and any specific experimental conditions.

Workflow: From Sample to Solution

Cell Isolation and Preparation

Regulatory T cells (Tregs) and responder T cells are meticulously isolated from supplied PBMCs (or other specified sources) and quality-controlled to ensure viability and purity.

Fluorescent Labeling

Responder cells are typically labeled with a proliferation-tracking dye to enable flow cytometric analysis of their division.

Co-culture Setup

Labeled responder cells and Tregs are co-cultured at various ratios in the presence of appropriate stimulation reagents (e.g., anti-CD3/CD28 antibodies or specific antigens) and the client's test molecules. This allows for the precise evaluation of Treg-mediated suppression.

Incubation

The co-cultures are incubated under optimized conditions for a predetermined period, typically 3-5 days, to allow for T cell activation and proliferation, and subsequent Treg suppression.

Flow Cytometric Analysis

Post-incubation, cells are harvested, stained for relevant surface markers (e.g., CD4, CD25), and analyzed by flow cytometry. This step accurately quantifies responder T cell proliferation and assesses Treg suppressive efficiency.

Service Highlights

Precise Quantification: Accurately measures Treg suppressive activity, providing quantitative data on immune modulation.
High Reproducibility: Optimized protocols ensure consistent and reliable results, critical for drug discovery and development.
Versatile Application: Applicable across various research areas, including autoimmunity, inflammation, infectious diseases, and oncology.
Cost-Effective: Offers an efficient and economical way to screen compounds and understand their immunomodulatory effects.
Customizable: Flexible assay parameters to meet your specific research needs and experimental designs.

FAQs

Q1: What types of cells can be used in Creative Biolabs' In Vitro Treg Suppression Assay?

A1: We can utilize a variety of cell sources, including fresh or cryopreserved human and mouse Peripheral Blood Mononuclear Cells (PBMCs), isolated CD4⁺ T cells, and specific Treg subsets. We also accommodate client-provided cells.
Q2: How is the suppressive capacity of Tregs measured in the assay?

A2: Treg suppressive capacity is typically measured by assessing the inhibition of the proliferation of responder T cells. We use advanced flow cytometry with proliferation dyes to precisely quantify cell division, providing clear, quantifiable suppression percentages.
Q3: Can Creative Biolabs customize the assay to fit my specific research needs?

A3: Absolutely. Our In Vitro Treg Suppression Assay is highly customizable. We can adjust cell ratios, stimulation methods, incubation times, and readouts to align perfectly with your unique experimental design and research objectives.

Related Services

To further support your immunology and drug discovery endeavors, Creative Biolabs offers a suite of complementary services:

Contact Us

Creative Biolabs is dedicated to providing cutting-edge solutions for your immunology and drug development research. Our In Vitro Treg Suppression Assay, combined with our extensive portfolio of related services, offers the precision, reliability, and expertise you need to accelerate your scientific breakthroughs. For more extensive information, please contact us or send us an inquiry.

Reference

Romano, Marco et al. "Past, Present, and Future of Regulatory T Cell Therapy in Transplantation and Autoimmunity." Frontiers in immunology vol. 10 43. 31 Jan. 2019, doi:10.3389/fimmu.2019.00043. Distributed under an Open Access License CC BY 4.0, without modification.