Creative Biolabs is one of the well-recognized experts that are professional in discovering potential antibody functions for a broad range of project objectives. With years of experience, our scientists can offer high-quality immune checkpoint functional assays to meet our clients’ demands precisely.
The immune checkpoint is a kind of signal for regulating the antigen recognition of T cell receptor (TCR) in the process of immune response. It can be divided into two types, namely co-stimulatory immune checkpoint and co-inhibitory immune checkpoint (Fig.1). Once the immune system is attacked by pathogens, these immune checkpoint molecules can protect the normal tissues from damage. Over the past several decades, multiple immunotherapy approaches have been developed with the aim to study the ability of an individual’s immune system to attack and destroy tumor cells. Particularly, antibody immunotherapies demonstrate great potential in eliciting durable disease control across a wide spectrum of cancer types, in contrast to the immediate but transient responses induced by conventional targeted therapies. With all these appealing features, immune checkpoint antibodies are gaining increasing popularity and attention as a promising solution for cancer treatment.
Fig.1 The blockade of immune checkpoints in cancer immunotherapy. (Pardoll, 2012)
With years of experience, we have established a novel platform which can offer a full range of immune checkpoint functional assays to discover your molecules’ potential to modulate immune function. We are happy to help global customers discover potential immune checkpoint inhibitors, especially antibodies. Based on our real-time, label-free platform, we are capable of providing multiple relevant assay endpoints to demonstrate the binding and functional properties of your molecule, along with its potential mechanism of action (MOA). The services we can offer include:
A number of agents can specifically or nonspecifically induce T cell activation, resulting in cytokine production, cytokine receptor expression, and ultimately proliferation of the activated T cells. T-cell proliferation assays are reliable, simple, and easy to perform and have been widely used to assess the activation of T cells. By real-time imaging T-cells' phenotype changes in response to antibody candidates, we can directly analyze its ability to restore T-cell functions. Meanwhile, detailed kinetic data and time-dependent effects can also be obtained, which are of great significance for future potency characterization.
Cytokine release syndrome (CRS) is a potentially adverse clinical event where proinflammatory cytokines (e.g. TNF-a, IL-6, IL-8, and IFN-c) are released from immune cells. CRS may occur on initial, and sometimes subsequent, intravenous infusion of some types of protein biotherapeutics. Based on our advanced technology, we offer high-quality in vitro cytokine release assay to predict the potential for a new biotherapeutic (also referred to as drug in this manuscript) to induce significant cytokine release from immune cells in vivo.
Cytotoxic T lymphocytes (CTLs) play an important role in cancer immunotherapies to eliminate tumor cells via vigorous immune responses. Creative Biolabs can analyze the potential of antibody candidates to modulate immune effector cells that kill tumor cells in a real-time, dye-free manner.
In addition, mixed Lymphocyte reactions (MLR) assays can also be used to understand the effects of biologics or small molecules on T cell activation, an important response in the tumor microenvironment (TME). Creative Biolabs is able to offer many different types of MLR assays, such as one-way, two-way, and three-way, to help global clients understand a drug candidate’s MOAs.
In vitro Treg suppression assay is one of the most widely used assays to test the suppressive capacity of Tregs. In order to better study the potential therapeutic reagents and related mechanism of T cells (Tregs) related action, Creative Biolabs has developed and optimized in vitro Treg suppression assays with quality-controlled Tregs, stimulation reagents, and responder cells.
Non-antigen specific functional assays have been used for in vitro pharmacodynamic evaluation, such as assessing the potential of T cell responses in biologic. Creative Biolabs is confident in providing in vitro non-antigen specific functional assay services to best suit your program requirements.
Immune Cells-Mediated Spheroid Tumor-Killing Assay (3D)
The establishment of a 3D spheroid tumor-killing assay platform is of great significance for cancer drug development. Creative Biolabs is committed to providing comprehensive immune cells-mediated spheroid tumor-killing assay (3D) service with higher quality to meet global customers' needs.
Antigen-Specific T-Cell Recall Assay
Creative Biolabs provides one-stop antigen-specific T-cell recall assay services to support global customers in the immune-oncology field. High-quality equipment, innovative technologies, and experienced scientists work together to make our services more flexible to meet clients' various needs.
Immune Synapse Formation Assay
Immune synapse formation assay is usually used for revealing the pathogenesis of disease and new drug development. Creative Biolabs provides high-quality immune synapse formation assay services to investigate IS formation and how various chemicals and therapies may influence it.
TDCC reporter assay is an innovative assay that utilizes reporter gene reaction to evaluate the efficiency of antibody drugs. Creative Biolabs delivers this assay for global customers to satisfy your diverse needs and speed up the development of drug discovery.
Immune Checkpoint Reporter Assay
In addition, Creative Biolabs developed a rapid and sensitive immune checkpoint reporter assay incorporating reporter gene reaction to screen or profile immune checkpoints, providing great convenience for cancer therapy development.
Taken together, our exhaustive service of immune checkpoint functional assays can guarantee our clients with the most compelling and reproducible outcomes, offering absolute confidence for subsequent investigations. Especially, we will try our best to meet every clients' guidelines, as well as the regulatory guidelines that they must follow. For more details, please feel free to contact us.
Reference
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