This assay measures the growth and division of T cells after they've been activated by a specific agent. By using real-time imaging, we can analyze how a drug candidate affects T cell function and gather valuable data on its kinetic and time-dependent effects.
Immune Checkpoint Functional Assay Services
Creative Biolabs is a recognized leader in discovering the functions of antibodies. Our experienced scientists specialize in high-quality immune checkpoint functional assays, providing precise and tailored services to meet a wide range of project goals.
Immune Checkpoints: A Promising Frontier in Cancer Treatment
Immune checkpoints are crucial signaling molecules that regulate T cell receptor (TCR) function during an immune response. They are categorized as either co-stimulatory or co-inhibitory, and their primary role is to protect healthy tissues from damage when the immune system is under attack. Over the past few decades, various immunotherapy strategies have been developed to harness the body's immune system to fight cancer. Among these, antibody-based immunotherapies have shown immense promise in achieving long-lasting disease control for many types of cancer, a significant improvement over the short-term effects of traditional targeted therapies. Due to these advantages, immune checkpoint antibodies are increasingly recognized as a powerful and promising approach to cancer treatment.
Fig.1 Impact of ICIs on T cells.1
Immune Checkpoint Functional Assays at Creative Biolabs
Drawing on our extensive experience, we've developed an advanced platform to provide a complete suite of immune checkpoint functional assays. This platform is designed to help you uncover the potential of your molecules to modulate immune function. We specialize in assisting clients worldwide in the discovery of promising immune checkpoint inhibitors, with a particular focus on antibodies. Using our real-time, label-free technology, we can generate a variety of assay endpoints to showcase the binding and functional characteristics of your molecule, as well as its potential mechanism of action (MOA).
Fig.2 Assay workflow.
Our Comprehensive Assay Capabilities
Cytokine Release Evaluation
Cytokine release syndrome (CRS) is a potentially dangerous side effect of some biotherapeutics, where immune cells release a flood of pro-inflammatory cytokines like TNF-α and IL-6. Our high-quality in vitro assay can predict if a new drug is likely to cause this significant cytokine release in a living organism.
T Cell Cytotoxicity Assay
This assay is used to study how cytotoxic T lymphocytes (CTLs) — immune cells that kill cancer cells — respond to a new drug. We analyze how an antibody candidate modulates these immune cells and their ability to kill tumor cells in a real-time, dye-free manner.
Mixed Lymphocyte Reaction (MLR) Assay
The MLR assay helps us understand how a drug, whether a small molecule or a biologic, affects T cell activation. This is an important process to study within the tumor microenvironment. We offer various types of MLR assays, including one-way, two-way, and three-way, to help our customers understand a drug candidate's mechanism of action.
In Vitro Treg Suppression Assays
This assay is a widely used method for testing the suppressive capacity of regulatory T cells (Tregs). We use high-quality Tregs, stimulation reagents, and responder cells to perform this assay, which helps us better study potential therapeutic agents and the mechanisms of T cell-related actions.
In Vitro Non-antigen Specific Functional Assays
These assays are used to evaluate how a biologic affects T cell responses in vitro. We provide non-antigen specific functional assay services to help with pharmacodynamic evaluation and meet your specific program needs.
Immune Cells-Mediated Spheroid Tumor-Killing Assay (3D)
Creating a 3D model for spheroid tumor-killing assays is crucial for developing cancer drugs. We offer a comprehensive service for immune cell-mediated spheroid tumor-killing assays in a 3D environment to help with cancer drug development.
Antigen-Specific T-Cell Recall Assay
We provide a comprehensive service for antigen-specific T-cell recall assays to support research in immune oncology. Our team of experienced scientists uses innovative technologies and high-quality equipment to provide flexible services that meet various customer needs.
Immune Synapse Formation Assay
The immune synapse is a critical junction between immune cells that is important for disease understanding and drug development. Our assay services help you investigate how the immune synapse forms and how different chemicals and therapies might influence it.
TDCC Reporter Assay
The TDCC reporter assay is an innovative method that uses reporter genes to measure the effectiveness of antibody drugs. We offer this assay to help our customers with drug discovery and development.
Immune Checkpoint Reporter Assay
We've developed a quick and sensitive immune checkpoint reporter assay that uses reporter genes to screen and profile immune checkpoints. This provides a great advantage for developing new cancer therapies.
Macrophage/Monocyte Inflammation Stimulation Assay Service
This assay helps us examine the ability of a compound to induce inflammation in macrophages and monocytes in a laboratory setting. We use advanced equipment and technologies to provide a flexible service that can meet diverse customer requirements.
CTL ELISPOT Assay
The CTL ELISPOT assay is a precise method for measuring the activation of cytotoxic T lymphocytes (CTLs) and evaluating CTL immunity. This assay is known for its superior sensitivity and efficient workflow, allowing us to provide flexible and tailored services to our customers.
Dendritic Cell Activation Assay
This cutting-edge assay is designed to assess the potential of therapeutic agents to trigger an immune response. We offer a variety of endpoint options and indicators, backed by advanced technology, to provide comprehensive and reliable results.
Immune Checkpoint-targeted Reporter Gene Assay
We have a complete range of immune checkpoint-targeted reporter gene assays, including co-stimulatory, inhibitory, myeloid cell, and bi-specific immune checkpoints. We also offer customized services for other targets, like cytokines and immune cells, to support therapeutic development with high accuracy.
Service Highlights
- High Precision: Accurate and reproducible measurements of immune modulation.
- Comprehensive Insights: Diverse readouts for a complete understanding of MOA.
- Accelerated Discovery: Rapid screening and characterization of drug candidates.
- Physiologically Relevant Data: Use of primary cells and advanced models for better translatability.
- Reduced Risk: Early identification of potential safety concerns and efficacy.
- Customizable: Tailored solutions to fit your unique project requirements.
Available Products at Creative Biolabs
Conveniently, we have developed a range of products to examine immune checkpoints' function, and we offer them to global customers to boost your drug discovery and development projects.
FAQs
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Q1: What kind of information can I expect from the assay results regarding my molecule's mechanism of action (MOA)?
A1: You'll receive detailed insights into your molecule's MOA through various functional readouts, including its impact on T cell proliferation, cytokine production, and specific immune cell interactions. We provide comprehensive data reports and scientific consultation to help you fully understand how your molecule modulates immune function.
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Q2: Are these assays suitable for both small molecules and antibody therapeutics?
A2: Yes, our Immune Checkpoint Functional Assays are versatile and optimized for characterizing both small molecule compounds and large biologics, including various antibody formats. Our platform is adaptable to a wide range of drug modalities and targets, ensuring we can support your unique project requirements. To learn more about how our assays can be tailored to your specific molecule, send us an inquiry.
Contact Us
At Creative Biolabs, we stand at the forefront of immunotherapy research, offering unparalleled expertise and cutting-edge platforms for Immune Checkpoint Functional Assays. With over two decades of experience, our commitment to scientific excellence and customer satisfaction sets us apart. Our integrated approach ensures that every assay is meticulously designed and executed, delivering robust and reliable data that you can trust. For more details of our immune checkpoint function assays, please feel free to reach out to us.
Reference
- Franzin, Rossana et al. "The Use of Immune Checkpoint Inhibitors in Oncology and the Occurrence of AKI: Where Do We Stand?." Frontiers in immunology vol. 11 574271. 8 Oct. 2020, doi:10.3389/fimmu.2020.574271. Distributed under an Open Access License CC BY 4.0, without modification.
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