Tumor cell proliferation assay is a valuable tool for studying the growth and viability of cancer cells in response to various treatments or experimental conditions. Luminescence-based assays are a highly sensitive and reliable method for detecting cell proliferation as they can detect even small changes in cell number or metabolic activity.

BT-474 Human Breast Cell

BT-474-based Proliferation Assay at Creative Biolabs

Our BT-474-based proliferation assay is a cutting-edge method used to measure the proliferation of tumor cells in human breast cells. The assay measures cell proliferation by quantifying the luminescent signal generated by ATP produced during cell metabolism. This highly sensitive and reliable assay allows for the rapid and accurate analysis of tumor cell proliferation.

Our BT-474-based proliferation assay service includes:

• Culturing and seeding of BT-474 cells in appropriate culture conditions.
• Treatment of cells with the test compounds or experimental conditions.
• Measurement of cell proliferation using a luminescence-based assay, such as ATP measurement or luciferase reporter assay.
• Data analysis and reporting of the results, including dose-response curves and IC50 values if applicable.

Assay Information

Assay Procedure

Our human breast cell BT-474-based proliferation assay typically follows the following process:

1. Culturing of BT-474 cells: BT-474 cells are cultured in appropriate media under optimal conditions to ensure their viability and proliferation.

2. Cell seeding: The cultured BT-474 cells are seeded into a 96-well plate at a specific density to ensure uniform distribution and accurate measurement of proliferation.

3. Treatment and incubation: The BT-474 cells are then treated with the compound or drug of interest at varying concentrations or with appropriate controls. The plate is then placed in an incubator for a specific duration to allow the cells to respond to the treatment

4. Luminescence measurement: After the incubation period, a luminescent reagent is added to the wells, which reacts with ATP present in metabolically active cells.

5. Data analysis: The luminescence readings are measured using a luminometer, and the data is analyzed to determine the effect of the treatment on cell proliferation. This may include calculating IC50 values, assessing dose-response curves, and determining the extent of cell growth inhibition or stimulation.

Advantages of BT-474 Human Breast Cancer Cell Line

BT-474 human breast cancer cell line is commonly used for tumor cell proliferation assay for several reasons:

• Relevance
  BT-474 cells are derived from a human breast cancer tumor, making them a relevant model for studying breast cancer cell proliferation.
• Hormone receptor status
  BT-474 cells are estrogen receptor-positive and HER2-positive, making them a suitable model for studying hormone receptor-positive breast cancers.
• Growth characteristics
  BT-474 cells have a rapid growth rate and form easily measurable colonies, making them ideal for cell proliferation assays.
• Genetic stability
  BT-474 cells are well characterized and have stable genetic characteristics, reducing variability in experimental results.
• Availability
  BT-474 cells are commercially available and widely used, making them a convenient choice for researchers conducting tumor cell proliferation assays.

Frequently Asked Questions

Q1: Applications of BT-474-based Proliferation Assay

A1:

• The BT-474-based proliferation assay can be used to screen potential anti-cancer drugs for their ability to inhibit the growth of breast cancer cells.
• Researchers can use the BT-474-based proliferation assay to study how different drugs or compounds affect the proliferation of breast cancer cells.
• By analyzing the response of BT-474 cells to different compounds, researchers can identify potential biomarkers that may predict treatment response or resistance in breast cancer patients.
• The BT-474-based proliferation assay can be used to assess the efficacy of combination therapies in inhibiting the growth of breast cancer cells.

Contact Us

Our experienced team of scientists can work with you to design and customize the assay to meet your specific research needs. With Creative Biolabs' expertise and state-of-the-art equipment, you can trust us to deliver accurate and reliable results in a timely manner. Contact us today to learn more about our tumor cell proliferation assay service using luminescence in BT-474 human breast cells and how we can help advance your research.

For Research Use Only | Not For Clinical Use