Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide CAL 27-based In Vitro Resazurin Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
CAL 27
Target Cell Organism
Human
Target Cell Background
Cal 27 cells are epithelial cells isolated from tissue taken prior to treatment from a 56-year-old, White male with a lesion in the middle of the tongue in 1982.
Related Diseases
Squamous Cell Carcinoma
Research Area
Oncology
Assay Name
In Vitro Resazurin Assay (Cell Viability)
Short Description
CAL 27-cell based In Vitro Resazurin Assay (Cell Viability)
Assay Description
Resazurin is a non-fluorescent (blue in color) component of alamar Blue reagents, which can enter viable cells. After entering in to viable cells, resazurin is converted to resorufin by NADH, NAD (P) H or FADH2-dependent cellular oxidore ductase.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins