Rhesus Monkey IFNA ELISA Kit, Lot 21AO-357 [Cancer Immune Checkpoint Assay Kit]

Instant ELISA Kit for Interferon Alpha (IFNa)

ELISA

Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
Kits from different batches may be a little different in detection range, sensitivity and color developing time.
There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.

Information on standard material: The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.
Information on reagents: The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.
Information on antibodies: The provided antibodies and their host vary in different kits.

IFNA

Rhesus Monkey

Colorimetric

Sandwich ELISA

Quantitative

This assay has high sensitivity and excellent specificity for detection of Instant Interferon Alpha (IFNa). No significant cross-reactivity or interference between Instant Interferon Alpha (IFNa) and analogues was observed.

Pre-coated, ready to use 96-well strip plate
Plate sealer for 96 wells
Standard
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30 x concentrate)
Instruction manual

Microplate reader with 450 nm filter.
Precision single or multi-channel pipettes and disposable tips.
Tubes for diluting samples.
Deionized or distilled water.
Absorbent paper for blotting the microtiter plate.
Container for Wash Solution

100 μL

1.5 h

Pre-coated

1.Prepare all reagents, samples and standards,
2.Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3.Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4.Aspirate and wash 3 times,
5.Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6.Aspirate and wash 5 times,
7.Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8.Add 50μL Stop Solution. Read at 450nm immediately.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

4 °C

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

6 months

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

For Research Use only

Interferon alpha

IFN-ALPHA-1; IFN1; Ifa; Ifa8; interferon; alpha 1; interferon alpha; interferon-alpha; IFNA1; Ifna; IFNA

JAK-STAT Signaling, TLR Signaling, Hepatitis C, Inflammasome

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interferon Alpha (IFNa). Standards or samples and HRP-labeled detection antibody specific to Instant Interferon Alpha (IFNa) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Interferon Alpha (IFNa), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm.