The in vitro validation study of oncolytic viruses is crucial. It can elucidate the biological and infectious characteristics, as well as the oncolytic mechanisms of virus products, ensuring virus quality. This provides a theoretical foundation for subsequent research and pre-clinical applications. Based on in vitro findings, researchers can rapidly screen for superior virus strains and optimize their working conditions. With extensive experience, Creative Biolabs offers professional and comprehensive in vitro validation services. These services accurately define the properties and functions of oncolytic viruses, facilitating more effective development and application of oncolytic virus-based therapies.
Quality testing of oncolytic viruses is crucial for ensuring their safety, efficacy, and batch-to-batch consistency. The following are some common quality-detection methods and corresponding detection indicators:
Target of detection | Experimental Methods |
---|---|
Morphology and structure | Electron microscopy |
Insoluble particles | Photoresistance method |
pH | pH determination method |
Capacity of loading | Weight/relative density method |
Purity | HPLC |
Sterility | Direct inoculation |
Endotoxin | Gel-clot method |
Mycoplasma | qPCR |
Culture method for mycoplasma detection | |
Indicator cell method |
The identity assay for engineered oncolytic viruses determines identity, characteristics, and purity. Analyzing pathogenic, trans, and viral-specific genes/proteins, ensures they meet quality standards for intended use.
Fig.1 Schematic diagram of the steps for plaque assay.1
Commonly used experimental methods include observation of cytopathic effect (CPE), neutral red uptake assay, MTT colorimetric method or other cell viability detection methods, colony formation assay, detection of cell apoptosis, determination of the ratio of viable and dead cells, etc.
After inserting the transgene into the oncolytic virus genome, qPCR and western blotting can be utilized to quantitatively and qualitatively analyze the transgene expression in the oncolytic virus. Additionally, immunological (immunohistochemistry, immunofluorescence, ELISA, etc.) and cell-biological techniques can be employed to determine its function.
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