With a proven reputation, innovative complement autoantibody test technology, high-quality assay results and a deep commitment towards addressing customers’ needs, Creative Biolabs provides a comprehensive range of complement autoantibody test services to advance your diagnostic and therapeutic discovery.
Background of C3 Autoantibody
C3 is a convergent point of the complement system, common between the classical, lectin, and alternative pathways. It is a complex protein, which generates different functional activated fragments (C3a, C3b, iC3b, C3c, C3d). These components play a critical role in orchestrating inflammatory and immune responses as well as in the clearance of immune complexes, but the autoreactivity to complement components may have considerable pathological consequences. Numerous studies show that autoantibodies against the central complement component C3 have been associated with many autoimmune diseases, such as SLE or Crohn disease, nephrotic kidney diseases and dense deposit disease (DDD), but their molecular mechanism is not well understood. To date, anti-C3 autoantibodies have been proven to inhibit the inactivation of C3b to iC3b by factor I in the presence of complement receptor 1 (CR1) as a cofactor and to prevent the clearance of apoptotic cells by mouse but not human macrophages.
Anti-C3 Autoantibody Test
Anti-C3 autoantibodies are usually measured through enzyme-linked immunosorbent assay (ELISA) with purified C3 immobilized on the microtiter plate. After antigen coating, the plates are blocked by the addition of PBS containing 0.25% Tween 20 and plasma is diluted 1:100 with the same solution. The binding of anti-C3 autoantibodies is determined by an anti-human IgG peroxidase-labeled antibody diluted 1:1000 in PBS containing 0.25% Tween 20, and a 3,3,5,5' tetramethylbenzidine substrate system. Alternatively, plasma samples are serially diluted starting from 1:50 and applied on coated and blocked plates to evaluate the dose-response of the binding of the autoantibodies.
Our Services of Anti-C3 Autoantibody Test
For more than a decade, we have successfully developed and qualified ELISAs for several purposes. As a one-stop-service provider, Creative Biolabs offers a wide range of complement test services to accomplish your development project. Our research scientists are professional in ELISA assay development and can assist clients based on their requirements. With our team of scientists' extensive experience in complement autoantibody test, we provide rapid, reliable, sensitive and cost-effective anti-C3 autoantibody test services and solutions.
Service highlight:
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Years of experience in the development of ELISAs against various targets
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Services from experienced and trustworthy scientists
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Guaranteed results with sensitivity & reproducibility and timely delivery
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Strong commitment to quality and service
We have abundant experience in developing various forms of testing methods. We can also work directly with you to design an assay that achieves your objectives. If you want to know more, please feel free to contact us.
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Questions & Answer
A: C3 plays a central role in the activation of the complement system. the activated form of C3 is the target of autoantibodies. Autoantibodies against the central complement component C3 are called anti-C3 autoantibody. IgG against C3b were discovered in the late twentieth century, when they were named immunoagglutinins. They are associated with the severity and onset of disease.
A: Anti-C3 autoantibodies have been detected in systemic lupus erythematosus (SLE), lupus nephritis (LN), Crohn's disease, some nephropathies, dense deposition disease (DDD), C3 glomerulopathy (C3G) and immune complex glomerulonephritis.
A: It is critical to determine whether anti-C3 autoantibodies are protective, disease-associated, or simply incidental. This is because anti-C3b autoantibodies have obvious functional consequences. More experimental studies are needed to confirm their role in disease pathogenesis and their relevance as biomarkers for clinical practice.
For Research Use Only.
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