Incorporating 2' NH2 modification into the ribose residue of the sugar-phosphate backbone is a common modification in the development of aptamers, which greatly increase the stability to nuclease. Creative Biolabs has engaged in aptamer identification and modification for many years and accumulated a lot of experience in this field. We provide a full range of flexible and reliable modification solutions to improve the resistance of aptamers to nuclease.
2' NH2 Modifications
Aptamers have great potential in the field of disease treatment and diagnosis. However, DNA or RNA aptamers are easily degraded by nucleases, which limits their therapeutic and diagnostic applications. Now more and more approaches have been developed to improving aptamer stability to nucleases including various linkage designs, modification strategies, and conjugation approaches. For modification strategies, the 2' sugar modifications with 2'-F, 2'-NH2, 2'-OMe, and LNAs are common. Aptamers chemically modified by NH2 in the 2' position significantly increase their nuclease resistance compared to unmodified aptamers. NH2 can be introduced into the 2'-position of the DNA or RNA oligonucleotide during library construction and at amplification steps in the selection process. The aptamers with nuclease resistance are selected by the SELEX process. Besides, NH2 can be also introduced into the aptamers selected by SELEX methods. However, modifying the aptamers in library construction and at amplification steps in the selection process is much easier when compared to post-SELEX modifications.
Fig. 1 2' NH2 modification.1
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We are a first-class service provider in the field of aptamer development. We offer a series of aptamer development services from library construction and aptamer selection to aptamer synthesis and validation. We aim to offer highly stable and reproducible aptamer products to support a variety of research applications such as disease treatment and diagnosis. To improve the stability of aptamers to nucleases, we also offer a series of modification strategies such as incorporation of 2'-NH2-modified nucleotides into aptamers.
Advantages
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Powerful technical platform to support one-stop aptamer development including modification, selection, identification, synthesis, and validation
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Highly experienced in-home scientists providing a variety of medication strategies to meet different requirements of different projects
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Stable and reproducible aptamer product delivery to support your project success
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Timely data feedback to facilitate the process of your project
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Best after-sales service
When you choose us, you will not only get highly stable aptamer products but also in-depth scientific support from experts with years of experience. If you are interested in our services, please feel free to contact us for more details.
Reference
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1.Lipi, Farhana, et al. "In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies." RNA biology 13.12 (2016): 1232-1245.
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Questions & Answer
A: NH2 can be introduced into the 2'-position of the DNA or RNA oligonucleotide during library construction and at amplification steps in the selection process. Aptamers have been selected from libraries where the 2'-hydroxyl (2'-OH) group of the pyrimidine is replaced by a NH2 group. Aptamers chemically modified by NH2 in the 2' position significantly increase their nuclease resistance compared to unmodified aptamers.
A: Compared to the unmodified aptamer, the 2' NH2 modified aptamer shows high affinity for the target, improved nuclease resistance and a longer half-life. This chemically modified aptamer is 100-fold more stable in serum than the natural aptamer.
A: Commonly applied targets include basic fibroblast, growth factor, human neutrophil, elastase, Fcε receptor I, cholin receptor, human interferon, etc.
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