This product is a 82 kDa recombinant human Complement Component C2 protein with a C-terminal 6-His tag expressed in the mouse myeloma cell line. It is available for Enzyme Activity Assays.
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| Expressed Host | Mouse myeloma cell line, NS0 |
| Specie Reactivity | Human |
| Target | C2 |
| Molecule Mass | 82 kDa |
| Purity | >95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining |
| Endotoxin | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Tris, NaCl and CaCl2 |
| Applications | Enzyme Activity Assays |
| Product Form | Liquid |
| Storage | Store at -20/-80°C. Aliquot to avoid repeated freezing and thawing. |
| Introduction | Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase. C2 is a major histocompatibility complex class-III protein. Selective cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to form C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to form C5a and C5b. |
| Alternative Names | Complement C2; Complement Component 2; C3/C5 Convertase; Complement Component C2; EC 3.4.21.43; ARMD14; CO2 |
| Gene ID | 717 |
| UniProt ID | P06681 |