This product is an unconjugated anti-Human Complement C1Q Polyclonal antibody generated from the Sheep. The antibody can be used for ELISA; FC; ID.
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| Clonality | Polyclonal |
| Host Animal | Sheep |
| Isotype | IgG |
| Immunogen | Human C1q, purified from plasma |
| Species Reactivity | Human |
| Applications | ELISA; FC; ID |
| Application Notes | ELISA: 1:100-1:2000 FC: 1:10-1:1000 The optimal working dilutions should be determined by the end user. |
| Specificity | This antibody reacts with Human complement C1Q. |
| Purity | ≥95% as determined by SDS-PAGE |
| Format | Liquid |
| Size | 100; 500 µL |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Type | Primary Antibody |
| Target Name | C1Q |
| Alternative Names | Complement C1q; C1q complex |
| Introduction | The complement component 1q (C1q) is a protein complex involved in the complement system, which is also a subunit of the first complement component C1. When C1q binds antigen-antibody complexes, the C1 complex becomes activated. Activation of the C1 complex initiates the classical complement pathway of the complement system. |
I have been using the antibody for ELISA assays, and the results have been consistently reliableExcellentThe antibody's binding affinity is excellent, providing clear and distinct signals in my assays. Additionally, the antibody shows minimal non-specific binding, which is crucial for the accuracy of my experiments.
The fluorescence intensity is robust, and the specificity towards C1Q is top-notchExcellentI appreciate the detailed datasheet provided, which guided me through the optimal dilution ratios and experimental conditions. It's definitely a staple in my lab now.
For immunodiffusion testExcellentThe precipitation lines are sharp and well-defined, indicating high specificity. The antibody has helped me generate reproducible results across multiple experiments. The product's packaging is also very secure, ensuring that the antibody remains intact during shipping.
Exceeded my expectations in ELISA applicationsExcellentIt delivers strong and consistent signals, making it easy to quantify the C1Q levels in my samples. The antibody's stability is impressive, with no noticeable degradation even after several freeze-thaw cycles.
As someone working on complement system research, this antibody has been invaluableExcellentIts performance in flow cytometry has been particularly noteworthy, providing clear discrimination between positive and negative populations. The antibody is easy to use and integrates seamlessly into my existing protocols.