Adenosine receptors (ARs) are a class of GPCRs that consist of four subtypes: A1, A2A, A2B, and A3. These receptors play a crucial role in mediating the physiological actions of adenosine. They are broadly identified in practically all human body tissues and organs, as well as their expression patterns vary among different cells, providing strength to the notion that they could potentially serve as both pathological indicators and unexpectedly pharmacological targets. Consequently, comprehending the function of adenosine receptors will boost their applications in drug development of diseases.
Creative Biolabs provides a cost-effective Magic™ in vitro cell-based adenosine receptor functional assay service to assist global customers in comprehending the function of adenosine receptors better. Our Magic™ in vitro cell-based adenosine receptor functional assay service is carried out by spotting the accumulation of cAMP in response to an increase in cAMP following the combination of adenosine receptors with the G protein.
Fig.1 Adenosine receptor agonists inhibit cAMP production to regulate physiological activity.1,3
Creative Biolabs provides extensively customized services covering cell culture to results analysis for global customers' projects implemented smoothly. Presented here is a simple workflow of our Magic™ in vitro cell-based adenosine receptor functional assay service.
Summary: This study explores the role of adenosine as an immunosuppressive agent that hinders the function of various immune cells, particularly T cells, by engaging the adenosine A2A receptor (A2AR). This action typically restricts the immune system's ability to combat tumors. Researchers utilized murine and human chimeric antigen receptor (CAR) T cells to illustrate that disrupting A2AR through a gene-editing approach with clinical potential significantly boosts these cells' effectiveness in living organisms, subsequently enhancing survival rates in mice. Given that A2AR activation results in increased levels of cAMP, the signaling molecule that mediates its inhibitory effects, the study assessed the impact of A2AR knockdown by measuring cAMP levels following NECA stimulation, underscoring the receptor's critical role.
Fig.2 The evaluation of cAMP level induced by A2AR knockdown.2,3
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Q1: What does the binding of adenosine to its receptors result in?
A1: When adenosine attaches to its receptors, brain activity slows and you experience sleepiness. Adenosine, therefore, promotes sleep and dilates blood vessels, most likely to ensure enough oxygenation while sleeping. Caffeine inhibits the adenosine receptor.References
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