The calcitonin receptor (CTR) belongs to a GPCR subfamily that contains multiple peptides. They are widespread throughout the life cycle of organisms. Although CTR is best recognized for its involvement in bone and calcium homeostasis, its expression has been associated with a variety of cancers, such as breast and bone tumors. Evidence indicates that CTR could function as a viable therapeutic target in cancer treatment. Thus, understanding the function of CTR has great significance for its application in drug development of cancer therapy.
Fig.1 The calcitonin receptor guards prevent bone loss and inflammation.1
Creative Biolabs offers a cost-effective Magic™ in vitro cell-based calcitonin receptor functional assay service to support global customers' projects. Our Magic™ in vitro cell-based calcitonin receptor functional assay service is carried out by measuring the calcium flux and cAMP accumulation in response to the cellular response triggered by the reaction of calcitonin receptors and their ligands. Our knowledgeable and skilled research teams enable us to provide our customers with comprehensive services, from the design of experiments to data presentation. The following is a simplified workflow of our Magic™ in vitro cell-based calcitonin receptor functional assay service.
Fig.2 The workflow of our Magic™ in vitro cell-based calcitonin receptor functional assay service.
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Various Cell Lines |
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Reliable & Reputable Experiment System |
| We provide multiple types of stable expressing cell lines including but not limited to human leukemia Jurkat T cells, human melanoma A375 cell lines, MCF-7 human breast cancer cells, CHO cell lines, etc., to choose from for global customers' projects. | Our experimental system has years of project experience and is continually being optimized as technology advances. Together with straightforward experimental procedures, we have complete confidence that we will present desirable results for global customers. | ||
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Competent Research Team |
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Full-around Services |
| Our research team features a group of specialists with extensive professional training, practical experience, and expertise, which together with state-of-the-art technologies make us confident in presenting high-quality data to global customers. | We provide an all-encompassing customized Magic™ in vitro cell-based calcitonin receptor functional assay service, from the preparation of samples to results presentation, incorporating the most up-to-date technological solutions. |
Fig.3 cAMP signaling by CTR activated by its cognate agonists.2
CTR function was assessed using a cAMP accumulation assay. The findings showed a significant, concentration-dependent rise in cAMP in response to CTR agonists in the traditional GBM model cell line SB2b. The strength of the reaction to sCT (pEC50 ± 9.4 0.3) and hCT (pEC50 ± 8.0 0.2) was comparable to their known CTR affinities.
For more details about our Magic™ in vitro cell-based calcitonin receptor functional assay service, please don't hesitate to contact us. We are confident in presenting ideal results for your project work with our top-ranking research team and abundant resources.
Q1: Which cells contain calcitonin receptors?
A1: Calcitonin receptors have been found in a variety of tissues throughout the body. The kidney, osteocytes, brain, testes, placenta, and lung all have receptors for calcitonin.
Q2: What is calcitonin like receptor CLR?
A2: CLR (calcitonin receptor-like receptor) does not bind any known endogenous ligand on its own, but when combined with RAMPs, it produces receptors for CGRP, adrenomedullin, and adrenomedullin 2/intermedin.
References
For Research Use Only.
