Hi-Affi™ In Vitro Cell based Calcitonin Receptor Functional Assay Service

Calcitonin Receptors with Prospect

The calcitonin receptor (CTR) belongs to a GPCR subfamily that contains multiple peptides. They are widespread throughout the life cycle of organisms. Although CTR is best recognized for its involvement in bone and calcium homeostasis, its expression has been associated with a variety of cancers, such as breast and bone tumors. Evidence indicates that CTR could function as a viable therapeutic target in cancer treatment. Thus, understanding the function of CTR has great significance for its application in drug development of cancer therapy.

Fig.1 The calcitonin receptor guards prevent bone loss and inflammation.¹

Our Hi-Affi™ In Vitro Cell-based Calcitonin Receptor Functional Assay Service

Creative Biolabs offers a cost-effective Hi-Affi™ in vitro cell-based calcitonin receptor functional assay service to support global customers' projects. Our Hi-Affi™ in vitro cell-based calcitonin receptor functional assay service is carried out by measuring the calcium flux and cAMP accumulation in response to the cellular response triggered by the reaction of calcitonin receptors and their ligands. Our knowledgeable and skilled research teams enable us to provide our customers with comprehensive services, from the design of experiments to data presentation. The following is a simplified workflow of our Hi-Affi™ in vitro cell-based calcitonin receptor functional assay service.

Fig.2 Our service process. (Creative Biolabs Original) Fig.2 The workflow of our Hi-Affi™ in vitro cell-based calcitonin receptor functional assay service.

The Breadth of Our Hi-Affi™ In Vitro Cell-based Calcitonin Receptor Functional Assay Service

	Various Cell Lines		Reliable & Reputable Experiment System
	We provide multiple types of stable expressing cell lines including but not limited to human leukemia Jurkat T cells, human melanoma A375 cell lines, MCF-7 human breast cancer cells, CHO cell lines, etc., to choose from for global customers' projects.		Our experimental system has years of project experience and is continually being optimized as technology advances. Together with straightforward experimental procedures, we have complete confidence that we will present desirable results for global customers.
	Competent Research Team		Full-around Services
	Our research team features a group of specialists with extensive professional training, practical experience, and expertise, which together with state-of-the-art technologies make us confident in presenting high-quality data to global customers.		We provide an all-encompassing customized Hi-Affi™ in vitro cell-based calcitonin receptor functional assay service, from the preparation of samples to results presentation, incorporating the most up-to-date technological solutions.

Representative data

Fig.3 cAMP signaling by CTR activated by its cognate agonists.²

CTR function was assessed using a cAMP accumulation assay. The findings showed a significant, concentration-dependent rise in cAMP in response to CTR agonists in the traditional glioblastoma multiforme (GBM) model cell line SB2b. The strength of the reaction to sCT (pEC50 ± 9.4 0.3) and hCT (pEC50 ± 8.0 0.2) was comparable to their known CTR affinities.

Work with Creative Biolabs

Contact flowchart. (Creative Biolabs Original)

For more details about our Hi-Affi™ in vitro cell-based calcitonin receptor functional assay service, please don't hesitate to contact us. We are confident in presenting ideal results for your project work with our top-ranking research team and abundant resources.

Frequently Asked Questions

Q1: Which cells contain calcitonin receptors?

A1: Calcitonin receptors have been found in a variety of tissues throughout the body. The kidney, osteocytes, brain, testes, placenta, and lung all have receptors for calcitonin.

Q2: What is calcitonin like receptor CLR?

A2: CLR (calcitonin receptor-like receptor) does not bind any known endogenous ligand on its own, but when combined with receptor activity-modifying proteins (RAMPs), it produces receptors for calcitonin gene-related peptide (CGRP), adrenomedullin, and adrenomedullin 2/intermedin.

References

Maleitzke, Tazio, et al. "The calcitonin receptor protects against bone loss and excessive inflammation in collagen antibody-induced arthritis." Iscience 25.1 (2022).
Distributed under Open Access License CC BY 4.0. The original image was modified by extracting and using a part, and the title was changed to "The calcitonin receptor guards prevent bone loss and inflammation".
Ostrovskaya, Anna, et al. "Expression and activity of the calcitonin receptor family in a sample of primary human high-grade gliomas." BMC cancer 19 (2019): 1-12.
Distributed under Open Access License CC BY 4.0. The original image was modified by extracting and using part C, and the title was changed to "cAMP signaling by CTR activated by its cognate agonists".