Custom Antisense Oligonucleotide Synthesis
Antisense oligonucleotides can act sequence specifically to modulate gene expression in living cells and thus are being considered for therapeutic application in diseases where the genetic target and its sequence have been identified. The antisense oligonucleotide therapeutics has been developed for the treatment of leukemias and viral infections such as HIV, herpes simplex, and human papillomavirus. Creative Biolabs offers reliable antisense oligonucleotide synthesis in ready-to-use duplex multi-scale quantities for your project development. These antisense oligonucleotides have been used as structural and mechanistic probes in diverse areas of diagnostic and therapeutic applications.
Antisense Oligonucleotides
Antisense oligonucleotides are synthetic polymers in which some or all of the natural nucleotide monomers of the oligonucleotide are chemically-modified deoxynucleotides (in DNA) or ribonucleotides (in RNA). Normally, antisense oligonucleotides contain 15 to 25 monomers. In antisense technology, single-stranded DNA or RNA molecules are used to target a specific sense mRNA. Antisense compounds have become effective tools for basic molecular biology, genomics, and proteomics research, which is often used for drug discovery, targeted screening, and validation. For example, antisense oligonucleotides can act by blocking the upstream message for receptor substrates, proteins over-expressed in pathological versus physiological states.
Figure 1. Comparison of antisense mechanisms.
Services
Creative Biolabs offers antisense oligonucleotides synthesis using DNA, 2'-O-Methyl RNA, RNA, or constrained nucleotide bases with either phosphorothioate or unmodified internucleotide bonds.
Figure 2. Selection of modified nucleic acid.
- Phosphorothioates
In this class of oligonucleotides, one of the non-bridging oxygen atoms is replaced by sulfur. Phosphorothioates are easy to synthesize, highly water-soluble and resistant against nucleolytic degradation.
- 2'-O-Methyl RNA and 2'-O-Methoxyethyl RNA
RNA derivatives with methyl or a methoxyethyl group at the 2'-position of the ribose have been used to obtain antisense oligonucleotides with improved properties. These modifications confer high nuclease resistance with reduced toxicity.
- Locked Nucleic Acids (LNA), Phosphorodiamidate Morpholino Oligomers (PMO) and N3'→P5' Phosphoramidates (NPs)
LNAs combine a number of desirable properties, including nuclease resistance and an unprecedented hybridization affinity towards complementary oligonucleotides. PMOs are resistant to nucleases and do not activate RNase H. NPs exhibit high affinity towards a complementary RNA strand and good nuclease resistance.
We also provide featured antisense oligonucleotides (ASOs) products with comprehensive plasmid backbones and gene modifications against a range of human diseases.
Creative Biolabs, a leading biology CRO, focuses exclusively on early drug discovery and development services. Relying on our state-of-the-art manufacturing facility and over years of combined experience in antisense oligonucleotide synthesis, we have delivered many antisense oligonucleotides that are supported by our in-house bioanalytical laboratory with a full spectrum of analytical tools capable of producing technically challenging yet safe and efficacious drug candidates. If you are interested in our services, please contact us for more details.
