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Custom miRNA Synthesis


MicroRNAs (miRNAs) are a class of small, non-coding double-stranded RNAs (dsRNAs) of 21-25 nucleotides in length, transcribed by the endogenous genome. They play a key regulatory role in distinct processes of organisms by targeting specific mRNAs for degradation or translation repression. Recent research advances have revealed the synthesis mechanisms and regulatory pathways of miRNAs in animals and plants.

Today, miRNA-based gene regulation is implicated in disease etiology and has been explored for treatment. As a reliable and skillful supplier in the world, Creative Biolabs devotes to the existing knowledge about miRNAs synthesis and is committed to preclinical and clinical trials that have been initiated for miRNA-based therapeutics. Here, we provide one-stop, customized miRNA synthesis services to create high-quality and high-bioactivity oligonucleotides for global scientists.

Functions of miRNAs

The core part of miRNA genes is a hairpin that eventually will come into being a ribonucleoprotein complex, which knocks down target genes’ expression through the identification and destruction of its transcript. Structural elements of this hairpin present a signal for processing by DROSHA and DICER, resulting in the formation of an approximately 20-23 bp miRNA duplex. The functional sequence of mature miRNA duplex is incorporated into the RNA-induced silencing complex (RISC), which promotes target mRNA recognition and ultimately cause gene knockdown. Synthetic miRNAs and by-products of the miRNA pathway, such as small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs), so far have been commonly used tools in molecular and cell biology.

Custom miRNA Synthesis Services at Creative Biolabs

miRNA biogenesis. Figure 1. miRNA biogenesis. (Rupaimoole, 2017)

The discovery and characterization of miRNA genes and their regulatory mechanisms not only provide a new understanding of physiological regulation of gene expression, but also open new opportunities for miRNA-based therapeutics. Recently, miRNA research has been promoted by technological advancements and is being investigated for its potential as a novel generation of drugs. Whether clients want to get fast protein expression by injecting or transfecting mRNA into cells as well as study regulatory miRNAs through profiling, overexpression, or knockdowns, Creative Biolabs is ready to support these studies with our high-quality custom miRNA synthesis services.

miRNA expression

Besides chemical synthesis, lentiviral vectors can also be used to express synthetic miRNA genes as genomic integrations of the transgene and long-term expression in target cells have been exhibited to be safe in patients. With further research, we’re able to optimize knockdown by synthetic miRNAs to the extent that permits competent therapeutic correction of pathological gene expression.

Hairpin architecture

The architecture of synthetic miRNAs, including the 3D structure of hairpins, is of great importance for the knockdown efficiency. Previous studies illustrated that the length of the lower stem is critical for effective processing by class 2 ribonuclease III enzyme (DROSHA) and the relative abundance of mature miRNA strands, leading to enhanced target gene knockdown. The architecture of miRNA genes is not limited to the hairpin. Other key parts contain promoters and nucleotide sequences not directly linked to the hairpin structure.

Concatenation strategy

Natural miRNA genes perform in a concatenated format. Their architecture is composed of an arrangement of some hairpins upon the control of a single promoter. Such concatenation is a powerful tool for biotechnology because multiple genes might be simultaneously targeted and knockdown potency can be increased by employing multiple miRNAs per target gene. Today, we have developed several concatenating strategies, including but not limited to:

The Items Need to Identify

Target Information Identify sense sequence (5'→3')
Identify antisense sequence (Optional, 5'→3')
3' Overhang Options Identify sense sequence (5'→3')
Identify antisense sequence (Optional, 5'→3')
Modifications Identify required modifications, such as biotin, phosphate, amino-modifier C7, 2'-OMe, 6-FAM, Cyanine 3, Cyanine 5, Cyanine 5.5, etc.
Purification Identify purification methods, such as HPLC, etc.
Quantities Identify required quantities, such as 50 nmol, 100 nmol, or 200 nmol.

Highlights

As a well-recognized service provider in oligonucleotide synthesis fields, Creative Biolabs is pleased to introduce a simple and convenient online ordering tool by which clients can enter mature miRNA sequences and choose any required chemical modifications to their miRNA products. Furthermore, we hold multiple scales, purification, and processing options for your special requests. If you don't find what you need on this webpage, please don’t hesitate to contact us for more information.

Reference

  1. Rupaimoole, R.; et al. (2017). MicroRNA therapeutics: towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 16(3): 203-222.

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