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Custom Circular Oligonucleotide Synthesis

Circular oligonucleotides are being studied vigorously as a new class of potential therapeutic agents. It was found that circular DNA or RNA oligonucleotides can inhibit the expression of specific genes. Creative Biolabs has focused on the development of oligonucleotide synthesis for many years and has established excellent platforms for drug development. We provide a variety of circular oligonucleotide synthesis services to meet the diverse needs of our customers.

Circular Oligonucleotides

Circular oligonucleotides essentially are duplex DNAs or RNAs that are capped by nucleotide loops on either end and often have high values of melting transition temperature. This strong secondary structure makes this kind of circle simpler to construct than those that lack a strong internal structure, because a linear circle precursor folds itself into the nicked dumbbell shape in solution. Circular oligonucleotides have a number of unusual and potentially advantageous properties. Many of these circular oligonucleotides have greater affinity, sequence selectivity and are more resistant to nucleases compared to their linear structures.

Circular oligonucleotides act as an effective DNA or RNA template to produce long, repeating copies of circular sequences in rolling circle PCR applications. Single-stranded circular DNA or circular RNA oligonucleotides are designed to undergo triplex formation with single-stranded DNA and RNA targets. The research shows that circular oligonucleotides bind a complementary strand of DNA more tightly than a standard Watson-Crick strand, moreover, its binding shows greater affinity than a singly linked, triplex-forming ligand. The strategies using circular oligonucleotide have shown interesting properties in diagnostic and therapeutic applications.

The strategy for forming triplex circular oligonucleotides. Figure 1. The strategy for forming triplex circular oligonucleotides.


The custom circular DNA or RNA oligonucleotides can be chemically synthesized at Creative Biolabs using the solution and solid-phase methods from partially modified linear precursors. After removing the linear DNA, circular oligonucleotides are purified by denaturing polyacrylamide gel electrophoresis (PAGE). Circularity is determined by exonuclease cleavage. At last, an independent QA procedure was applied to guarantee the highest quality for each circular oligonucleotide. Synthetic circular DNA or RNA oligonucleotides are employed in a variety of molecular biology applications such as real-time PCR, site-directed mutagenesis, microarrays, and various therapeutic applications.


Creative Biolabs’ custom circular oligonucleotide synthesis services offer numerous options to meet a variety of research needs. Our team provides you with outstanding support and meets your specific needs with a professional technology platform. If you are interested in our services, please contact us for more details.

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