3D Ex Vivo Human Ovary Clear Cell Adenocarcinoma Model Introduction

Creative Biolabs aims to provide reliable 3D ex vivo human ovary clear cell adenocarcinoma model to meet the needs of scientific researchers and drug developers. This helps drive further optimization of the understanding and treatment strategies for ovary clear cell adenocarcinoma.

What is Ovarian Clear Cell Adenocarcinoma (OCCA)?

Ovarian clear cell adenocarcinoma (OCCA) originating from the Müllerian ducts is a rare and aggressive ovarian epithelial malignancy.

Incidence: OCCA is relatively rare relative to other ovarian cancer subtypes, accounting for approximately 5-10% of all ovarian cancers. OCCA is more common in perimenopausal women who are nearly 50 years old or older.

Clinical manifestations: abdominal pain, pelvic pressure, abdominal mass, irregular vaginal bleeding, etc.

Pathological features: OCCA mostly occurs in the unilateral ovary. OCCA is usually a large, adenofibromatous mass that usually consists of a unilocular or multilocular thick-walled cyst containing a watery or myxoid fluid. Multiple histological structures, including solid, tubular cystic, and papillary, often co-exist in the tumor body of OCCA. Hyalinization of the nipple shaft or stroma is typical of OCCA.

Fig 1. Histopathological features of clear cell carcinoma. (Iida, et al., 2021)

Morphological characteristics of cells: OCCA cells are in various shapes, such as shoe-shaped, polygonal, cubic, columnar, and flat, with transparent cytoplasm. Among them, the hobnail-shaped cells with large and deeply stained nuclei are located at the top of the cytoplasm and protrude toward the lumen, which are the hallmark cells of OCCA.

Immunophenotype: positive for carbohydrate antigen 125 (CA125), NapsinA, and hepatocyte nuclear factor 1β (HNF1β), negative for Wilm tumor 1 gene (WT-1) and estrogen receptor (ER).

Prognosis and treatment: OCCC has a poor prognosis relative to other ovarian cancer subtypes. It is usually more aggressive and insensitive to chemotherapy. Surgical resection is the main treatment, but the postoperative recurrence rate is high. Therefore, the development of targeted therapeutic drugs is urgently needed.

3D Ex Vivo Human OCCA Model for Targeted Therapy Development

With years of experience and advanced platform, Creative Biolabs offers customized construction of 3D ex vivo human OCCA models to meet every client's specific requirement. Compared to traditional 2D cell culture models and animal models, this model can better simulate OCCA cells and their microenvironment within the human body. It can be used for the following research purposes:

Drug screening

By culturing a large number of 3D ex vivo human OCCA models in high-throughput culture systems, candidate drugs can be screened and evaluated to identify more effective treatment strategies.

Anti-tumor assessment

By simulating the human tissue environment, candidate drugs can be more accurately assessed for killing tumors, providing reliable data support for preclinical studies.

Disease mechanism research

The 3D ex vivo human OCCA model can simulate the development process and pathological features of OCCA, aiding in the in-depth study of disease mechanisms, signaling pathways, and targets and providing new clues for the diagnosis and treatment of the disease.

Construction of 3D Ex Vivo Human OCCA Models

Tissue collection: OCCA tissues are collected from patients with appropriate permissions.
Tissue culture: Based on research requirements, suitable 3D tissue culture techniques are chosen, and the models are maintained and cultured in appropriate culture systems to ensure stability.
Drug addition: Upon receiving the client's candidate drugs for testing, different concentrations of the drugs are added to treat the models.
Functional and characterization analysis: The constructed 3D ex vivo human OCCA models are undergone functional and characterization analysis, including assessments of cell proliferation, apoptosis, migration, invasion capabilities, etc.
Application and evaluation: Relevant assessments and data analysis are performed based on specific research objectives.

Creative Biolabs prioritizes quality control of 3D ex vivo models to ensure the stability and reliability for our model. If you have been seeking the optimal OCCA model for preclinical drug development applications, we encourage you to contact us for more detailed information about our 3D ex vivo human OCCA models. Furthermore, we also offer other types of 3D isolated human ovarian tissue models listed below.

Reference

Iida, Y.; et al. Clear cell carcinoma of the ovary: a clinical and molecular perspective. Int J Gynecol Cancer. 2021, 31(4): 605-616. Distributed under Open Access License CC BY 4.0. The original image was modified by keeping part (A) & (B).

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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