This product is an aptamer which binds to the cAMP with an affinity of <10 µM.
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| Target Category | Small Organic |
| Target | cAMP |
| Alternative Names | Cyclic adenosine monophosphate; cyclic AMP |
| Target Overview | Cyclic adenosine monophosphate (cAMP, cyclic AMP, or 3',5'-cyclic adenosine monophosphate) is a second messenger important in many biological processes. cAMP is a derivative of adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, conveying the cAMP-dependent pathway. It should not be confused with 5'-AMP-activated protein kinase (AMP-activated protein kinase). |
| Chemical Modification | RNA |
| Length | 20-40 nt |
| Affinity (Kd) | <10 µM |
| Binding Conditions/Buffer | 20 mM Tris-HCl (pH 7.5 at 23 °C), 450 mM NaCl, 100 mM KCl, 10 mM MgCl2, 1 mM MnCl22, and 5 mM CaCl22. |
| GC Content | 0.5161 |
| Molecular Weight | 10044.17 g/mole |
| Extinction Coefficient | 316000 L/(mole·cm) |
| nmoles/OD260 | 3.16 |
Superior in terms of performance and ease of useExcellentThis aptamer is a gem! It effectively binds to cAMP with minimal background noise, allowing for precise measurements even at low concentrations.
Highly recommended for anyone studying cyclic AMPExcellentWe switched to the anti-cAMP aptamer from traditional antibody-based assays and haven't looked back since. Its sensitivity and reliability have set a new standard in our lab.
It's now our go-to choiceExcellentThe aptamer is a must-have for anyone studying GPCR signaling pathways. It's robust and reproducible, providing consistent results across different experimental conditions.
A crucial tool in our research toolkitExcellentWe integrated this aptamer into our biosensor development project and were amazed by its performance. It's highly sensitive and ideal for real-time monitoring applications.