Creative Biolabs is a leading service company supporting clinical and pre-clinical research in the aptamer field. To bring our customers impeccable service of aptamer function validation, the seasoned scientific team from Creative Biolabs has developed an integrated aptamer cytotoxicity assay platform to test aptamer cytotoxicity comprehensively.
Aptamer is a new class of small multifunctional ligands comprising short single-stranded oligonucleotides with high affinity and specificity for their targets. The targets of aptamers range from metal ions to small molecules, proteins, and even mammalian cells. Aptamer is developed as effective targeted drug delivery systems to reduce or eliminate the severe side effects of chemotherapy as well as molecular recognition probes for cancer detection. These drug-delivery systems that specifically recognize targets will minimize side effects caused by nonspecific drug toxicity.
Aptamer Cytotoxicity Assays at Creative Biolabs
Aptamer cytotoxicity assays provide tremendous opportunities for refining and improving the design of the aptamer for application in vivo. Thus, these approaches might find potential applications in new drug development, existing drug improvement, and drug delivery for therapy. In order to investigate possible reasons for the selective cytotoxicity of aptamers, different types of cytotoxicity assays can be designed and performed in Creative Biolabs:
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MTS Assay
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MTT Assay
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Lactate Dehydrogenase (LDH) Assay
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In Vitro Annexin V Cytotoxicity Assay
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The in vitro specific cytotoxicity of the aptamer can be measured using MTS assay. The cytotoxicity assay will be performed in triplicate, and the equation will calculate the cell survival in each group. Due to the MTS assay only measured for cell metabolic activity, flow cytometry will be utilized to ascertain the selective cytotoxicity of aptamers.
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The MTT assay indirectly assesses the ability of the aptamers to accumulate in the target cells and healthy cells. MTT assay provides data on cell viability, specifically the cell metabolic activity.
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LDH activity in the aptamer treatment can be also measured using the LDH cytotoxicity assay kit. The percentage of cellular cytotoxicity was computed by calculating the total level of LDH released into the culture supernatant. LDH assay is a measure of cytotoxicity induced by cell membrane damage.
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To examine whether the selective delivery would result in targeted cytotoxicity, the annexin V assay can be performed to compare the cytotoxic effects of free aptamer on both target cells and control cells in vitro by performing.
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Creative Biolabs will accelerate the development and potential application of aptamers with our robust aptamer cytotoxicity assays. Based on the outcomes of these assays, the cytotoxic concentration of the present aptamers can be obtained, and these aptamers will be used for the remaining functional assays.
With our profound knowledge in functional aptamer mechanisms, Creative Biolabs introduces our world-class platform of aptamer cytotoxicity assay for our customers all over the world. Our researchers have earned an excellent reputation from our clients for superior aptamer cytotoxicity assays. We also guarantee friendly and professional post-sale services if you encounter any questions. Please feel free to contact us for more information.
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Questions & Answer
A: The cytotoxicity of aptamers can be influenced by factors such as their sequence, length, modifications, concentration, and the specific target they bind to.
A: When interpreting cytotoxicity assay results for aptamers, it is important to consider the assay method used, the concentration range tested, the specific cell type used, and whether the observed cytotoxicity is due to nonspecific effects or specific interactions between the aptamer and its intended target.
A: Some of the main challenges in evaluating the cytotoxicity of aptamers include designing appropriate controls, selecting relevant cell types, optimizing assay conditions, and interpreting the results in the context of aptamer concentration and exposure duration.
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