Aptamers, commonly known as synthetic antibodies, are single-stranded DNA or RNA molecules that fold into defined secondary structures and bind to the target with high affinity and specificity. Aptamers have many attractive properties, including rapid blood clearance and tumor penetration, and are promising candidates for molecular imaging applications. At present, scientists carry out many studies on the structure-activity relationship (SAR) of aptamers to improve the stability and targeting ability of aptamers.
Background of Stability Analysis for Aptamer
There is increasing interest in using aptamers to develop therapeutic and diagnostic agents. However, as oligonucleotides, aptamers are susceptible to nuclease degradation, which may affect in vivo functions. Considering that the conformation of nucleic acids is mainly based on the interaction between nucleotides, the composition of nucleotides and dinucleotide may affect the specific three-dimensional structure and stability of aptamers. The stability of an aptamer is a function of structure and chemical composition, and a stable and target-specific spatial conformation is the basis of the function of the aptamer. Therefore, it is very necessary to conduct in vitro stability evaluation experiments on aptamers.
Stability Analysis Services for Aptamer
The thermal stability and conformational stability of aptamers and aptamer-target complexes are important for field-based diagnostic analysis and biosensing applications. Currently, various tools and techniques are available to improve the stability of aptamers. Improved conformational stability and promotion of double-stranded conformation can provide enhanced nuclease resistance, thereby optimizing and broadening the application range of aptamers.
Fig.1 Various modifications of aptamer to increase its stability and potential applications. (Han, 2020)
Understanding the stability of the aptamer and determining the best option to enhance stability is a key part of the project design process. The required stability and potential modifications must be carefully considered. In addition to obtaining the required stability, the best method will retain the selectivity and function of the aptamer while minimizing the cost and time of aptamer production. Therefore, Creative Biolabs provides in vitro aptamer stability analysis services, including nuclease stability, biological stability, structural stability analysis, and thermal stability analysis.
Why Choose Us?
After more than 10 years of experience, Creative Biolabs is the best choice for high-quality, cost-effective outsourcing/collaboration options for your research projects. Our services cover all aspects of aptamer development, including aptamer screening, aptamer-based conjugation, aptamer engineering modification, aptamer characterization and in vitro analysis. We understand that every customer has different requirements for their projects, and we are happy to work with you to design a selection process that best suits your needs. If you have any service issues, please contact us.
References
-
From Wikipedia: By Fdardel - Own work, CC BY-SA 3.0， https://commons.wikimedia.org/wiki/File:Aptamer_biotin.png
-
Han, Jing, et al. "Application and development of aptamer in cancer: from clinical diagnosis to cancer therapy." Journal of Cancer 11.23 (2020): 6902.
Related Product
Questions & Answer
A: Several factors can influence aptamer stability, including temperature, pH, ionic strength, enzymatic degradation, and exposure to nucleases or other degrading agents.
A: Aptamer stability analysis typically involves studying the aptamers under different conditions, such as varying temperatures, pH levels, and ionic strengths. Techniques such as gel electrophoresis, surface plasmon resonance (SPR), and fluorescence-based assays can be used to evaluate stability.
A: Aptamer stability analysis provides valuable information about the factors affecting aptamer stability. By understanding the conditions that lead to aptamer degradation, researchers can make predictions and recommendations for optimizing storage conditions and estimating shelf life.
For Research Use Only.
Related Sections:
