B Cell-based Western Blot Assay

Introduction of Western Blot

Western blot (WB) is a widely used technique to isolate and identify specific proteins in a protein mixture extracted from cell or tissue lysates based on the merits of specific antibodies. In this technique, protein samples are loaded on an electrophoresis gel and separated by molecular weight. The gel is then transferred to PVDF under electricity to produce corresponding bands, as shown on the SDS-PAGE gel. After a blocking step, the membrane is then probed with antigen-specific primary antibody, and bound Abs are detected with enzyme-conjugated secondary antibody. Due to the specificity of the antibody, only one band indicating the targeted antigen can be visible. The targeted protein is quantified by comparing the thickness of the band to a reference protein band.

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Fig.1 Examples of colorimetric, radioactive, and fluorescent detection using an indirect method and a direct method. (OA Literature)

Fig.1 Multiple methods for detection and subsequent visualization of Western Blots.¹

What We Can Offer

Multiple western blot methods	Application of WB in Cancer Epitope Analysis
Radioactive Western Blot Fluorescent Western Blot Colorimetric Western Blot	Identify the shortest epitope reactive to specific antibodies. Provide a comprehensive epitope mapping of a specific antigen. Western blot can be used in combination with immunoprecipitation to examine the precipitated antigen peptide.

Highlights

Unparalleled Precision

Partnering with Creative Biolabs provides a team that delivers unparalleled precision and efficiency in antibody discovery. We leverage the power of the body's natural processes to streamline your research and development, ensuring a faster path to meaningful results.

Superior Specificity

Our western blot assay stands out by leveraging the body's natural selection process. This approach results in antibodies with superior specificity, designed to recognize their targets with high-fidelity accuracy and function.

Reduced Off-Target Binding

The B cell-based approach minimizes the risk of off-target binding and significantly reduces background noise. This leads to cleaner, more reliable data for your experiments, allowing you to interpret results with greater confidence and accuracy.

Published Performance

Published data consistently show the superior performance of our methods compared to conventional techniques. This evidence-based approach gives you confidence in the quality and reliability of our services, ensuring your project's success.

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Customer Reviews

Exceptional Efficiency
The streamlined workflow and rapid turnaround from Creative Biolabs' service facilitated a huge reduction in the time we normally spend on screening, allowing our team to focus on downstream applications. - M**ra F**z, Principal Investigator

Clarity in a Complex Field
We compared their service to traditional methods and found a drastic reduction in background noise. This clarity was instrumental in our discovery of a new low-abundance biomarker for a complex disease. - D**na S**h*, Senior Research Fellow.

FAQs

What types of samples can be used for the B-cell-based western blot assay?

We can work with a variety of samples, including peripheral blood mononuclear cells (PBMCs), splenocytes, or bone marrow from human or animal donors, allowing for flexible project design.

Can your service help with identifying antibodies against post-translational modifications (PTMs)?

Yes, our assay is uniquely suited to identify antibodies that recognize specific PTMs. By screening a native repertoire of B cells, we can discover antibodies that bind to a protein in its biologically relevant form.

Related Services

Epitope Binning and Mapping

A crucial step in antibody development, this service groups antibodies that bind to the same or overlapping epitopes, providing critical information for intellectual property and therapeutic development.

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Protein Purification

Achieve high purity and yield for your target proteins through our advanced chromatography-based purification services. This is a critical step for preparing high-quality antigens for your Western Blot projects.