Recombinant Anti-HER2 x Anti-VEGF Bispecific Antibody (scFv-IgG) is formed by an IgG and two scFv fragments. The scFvs from an anti-HER2 antibody variable domain are respectively connected to the N terminus of the anti-VEGF IgG heavy chains or light chains to form scFv-(H)IgG or scFv-(L)IgG. It is a type of appended IgGs and has 2+2 antigen-binding valency. This BsAb can bind two factors simultaneously and inhibit cell proliferation. It is designed for the research of Colorectal cancer; Breast cancer; Lung cancer; Renal cell cancer (RCC); Glioma cancer; Glioblastoma; Ovarian cancer therapy.