B Cell based Calorimetry Assay Service

Creative Biolabs' B cell-based calorimetry assay provides a powerful solution for accelerating your project with precise, actionable data. You can expect a clear understanding of B-cell functional states, including proliferation, activation, and metabolic shifts, all in a label-free environment. This allows for superior characterization of cellular responses in contexts like infectious disease research, autoimmune studies, and oncology.

Principal of the Calorimetry Assay

In general, the interaction process between biomolecules often causes heat release or heat absorption. The isothermal titration calorimetry (ITC) assay is used to directly measure the amount of heat released or absorbed between protein and protein interactions in solution, which is convenient and widely applied to quantify the binding affinity. The experiment is carried out in an isothermal titration calorimeter at a constant temperature.

Discover how we can help - Request a consultation.

Fig.1 Schematic diagram of an isothermal titration calorimetry instrument. ¹

What We Can Offer

• Data Interpretation
  The selective and specific binding between an epitope with an antibody is identified by comparing the K_D value.

  KD	Affinity
  nM, 10-9 M level	Strong
  μM, 10-6 M level	Medium-strength
  mM, 10-3 M level	Weak

Calorimetry Assay Services at Creative Biolabs

Highlights

Unmatched Versatility

Our ITC assay is designed to be highly versatile, capable of measuring interactions between a wide variety of biomolecules, including proteins, nucleic acids, and lipids. This adaptability makes it an essential tool for diverse research and drug discovery projects.

Label-Free Advantage

All samples are prepared in solution, eliminating the need for any chemical labeling. This ensures that the binding interaction is measured in its native state, providing you with a more accurate and biologically relevant representation of the molecular event.

Minimal Sample Requirement

The ITC assay requires only a minimal amount of reagent to develop the binding assay. This is particularly beneficial for studies involving rare or expensive materials, allowing you to conserve your valuable samples while still obtaining high-quality data.

Direct Binding Measurement

Our platform directly measures binding affinity across a broad range, from sub-millimolar to nanomolar. This direct measurement provides an unambiguous value for the binding strength, which is crucial for lead optimization and candidate selection.

Discover how a partnership with us can streamline your research - Get a quote today.

Customer Reviews

• Streamlined Antibody Screening
  Creative Biolabs' platform facilitated a direct comparison of B cell responses to various antigens, providing a clear metabolic fingerprint for each. This helped us streamline our therapeutic antibody selection process and reduce our timeline. - Dr. La Sz.
• Optimized Therapeutic Dosing
  We were able to monitor the effects of an immunosuppressant on B-cell metabolism with unprecedented detail. The data allowed us to optimize our dosing strategy and validated our in vitro findings against in vivo models. - Dr. Pl Bs.

FAQs

Related Services

How to Contact Us

Creative Biolabs provides trusted services to accelerate your cancer research and drug development. We will work closely with our partner to outline your specific requirements and objectives and fulfill them on time. For further details, please don't hesitate to contact us.

Reference

1. Chung, Chun-wa. "The use of biophysical methods increases success in obtaining liganded crystal structures." Acta crystallographica. Section D, Biological crystallography vol. 63, Pt 1 (2007): 62-71. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.1107/s0907444906051869

Download our brochure