B Cell based Calorimetry Assay Service
Creative Biolabs' B cell-based calorimetry assay provides a powerful solution for accelerating your project with precise, actionable data. You can expect a clear understanding of B-cell functional states, including proliferation, activation, and metabolic shifts, all in a label-free environment. This allows for superior characterization of cellular responses in contexts like infectious disease research, autoimmune studies, and oncology.
Introduction What We Can Offer Workflow Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Principal of the Calorimetry Assay
In general, the interaction process between biomolecules often causes heat release or heat absorption. The isothermal titration calorimetry (ITC) assay is used to directly measure the amount of heat released or absorbed between protein and protein interactions in solution, which is convenient and widely applied to quantify the binding affinity. The experiment is carried out in an isothermal titration calorimeter at a constant temperature.
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Fig.1 Schematic diagram of an isothermal titration calorimetry instrument. 1
What We Can Offer
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Animal Models Available
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Capabilities of ITC Assay
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Vicugna pacos
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Lama glama
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Mouse
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Camel
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Measure quantitative and qualitative antibody-antigen binding interaction.
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Determine association and dissociation constants.
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Assess binding affinity using full-length or fragment antibodies.
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Modify binding stoichiometries to understand interaction thermodynamics.
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Data Interpretation
The selective and specific binding between an epitope with an antibody is identified by comparing the KD value.
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KD
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Affinity
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nM, 10-9 M level
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Strong
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μM, 10-6 M level
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Medium-strength
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mM, 10-3 M level
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Weak
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Calorimetry Assay Services at Creative Biolabs
Highlights
Unmatched Versatility
Our ITC assay is designed to be highly versatile, capable of measuring interactions between a wide variety of biomolecules, including proteins, nucleic acids, and lipids. This adaptability makes it an essential tool for diverse research and drug discovery projects.
Label-Free Advantage
All samples are prepared in solution, eliminating the need for any chemical labeling. This ensures that the binding interaction is measured in its native state, providing you with a more accurate and biologically relevant representation of the molecular event.
Minimal Sample Requirement
The ITC assay requires only a minimal amount of reagent to develop the binding assay. This is particularly beneficial for studies involving rare or expensive materials, allowing you to conserve your valuable samples while still obtaining high-quality data.
Direct Binding Measurement
Our platform directly measures binding affinity across a broad range, from sub-millimolar to nanomolar. This direct measurement provides an unambiguous value for the binding strength, which is crucial for lead optimization and candidate selection.
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Customer Reviews
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Streamlined Antibody Screening
Creative Biolabs' platform facilitated a direct comparison of B cell responses to various antigens, providing a clear metabolic fingerprint for each. This helped us streamline our therapeutic antibody selection process and reduce our timeline. - Dr. La Sz.
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Optimized Therapeutic Dosing
We were able to monitor the effects of an immunosuppressant on B-cell metabolism with unprecedented detail. The data allowed us to optimize our dosing strategy and validated our in vitro findings against in vivo models. - Dr. Pl Bs.
FAQs
How does B Cell-based Calorimetry compare to flow cytometry?
Flow cytometry is excellent for phenotyping, but our calorimetry assay provides a direct, label-free measure of a cell's dynamic functional state. It gives you a continuous data stream, revealing real-time metabolic shifts that are invisible to a single-time-point flow cytometry measurement.
Is this assay suitable for high-throughput screening?
Yes. Our platform is optimized for high-throughput applications, making it ideal for screening multiple samples and conditions in parallel. This can significantly accelerate your research and drug discovery efforts.
Related Services
T and B Cell-based Surface Plasmon Resonance (SPR) Assay
A label-free, real-time method for quantifying binding interactions between cellular receptors and ligands, providing critical kinetic and affinity data for drug discovery and epitope analysis.
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ADC Antibody Screening
Our specialized service to identify and optimize antibodies for Antibody-Drug Conjugate (ADC) development, focusing on high specificity, affinity, and internalization for targeted therapy.
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How to Contact Us
Creative Biolabs provides trusted services to accelerate your cancer research and drug development. We will work closely with our partner to outline your specific requirements and objectives and fulfill them on time. For further details, please don't hesitate to contact us.
Reference
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Chung, Chun-wa. "The use of biophysical methods increases success in obtaining liganded crystal structures." Acta crystallographica. Section D, Biological crystallography vol. 63, Pt 1 (2007): 62-71. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.1107/s0907444906051869
For Research Use Only | Not For Clinical Use
